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Isolation of primary mice splenocytes for in vitro research

Fawzi O. Ebrahim, Abdul M. Abolayha, Adam A. Elzagheid, Fadella Abosrer.

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  • Abstract
    Abstract
    Animal cell culture is of great importance in research. We isolated mouse splenocytes from Swiss albino mice by using a mechanical method and cultured them in medium with fetal bovine serum, 1% penicillin-streptomycin and 1% amphotericin as an antibiotic and an antifungal, but without mitogen. The cells were confluent after 72 h, and their viability, measured by trypan blue assay, was 95%. The splenocytes were passaged four times and no fungal or bacterial contamination was observed during carrying out the experiment.

    Key words: Key words: mouse spleen, splenocytes, trypan blue


     
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    How to Cite this Article
    Pubmed Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. 2020; 64(2): 13-20. doi:10.5455/ajvs.82519


    Web Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. http://www.alexjvs.com/?mno=82519 [Access: August 17, 2021]. doi:10.5455/ajvs.82519


    AMA (American Medical Association) Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. 2020; 64(2): 13-20. doi:10.5455/ajvs.82519



    Vancouver/ICMJE Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. (2020), [cited August 17, 2021]; 64(2): 13-20. doi:10.5455/ajvs.82519



    Harvard Style

    Ebrahim, F. O., Abolayha, . A. M., Elzagheid, . A. A. & Abosrer, . F. (2020) Isolation of primary mice splenocytes for in vitro research. AJVS, 64 (2), 13-20. doi:10.5455/ajvs.82519



    Turabian Style

    Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. 2020. Isolation of primary mice splenocytes for in vitro research. Alexandria Journal of Veterinary Sciences, 64 (2), 13-20. doi:10.5455/ajvs.82519



    Chicago Style

    Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. "Isolation of primary mice splenocytes for in vitro research." Alexandria Journal of Veterinary Sciences 64 (2020), 13-20. doi:10.5455/ajvs.82519



    MLA (The Modern Language Association) Style

    Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. "Isolation of primary mice splenocytes for in vitro research." Alexandria Journal of Veterinary Sciences 64.2 (2020), 13-20. Print. doi:10.5455/ajvs.82519



    APA (American Psychological Association) Style

    Ebrahim, F. O., Abolayha, . A. M., Elzagheid, . A. A. & Abosrer, . F. (2020) Isolation of primary mice splenocytes for in vitro research. Alexandria Journal of Veterinary Sciences, 64 (2), 13-20. doi:10.5455/ajvs.82519





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