E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources

Eman M. Omr And Eman E. Abdeen.


Abstract
Pathogenic Escherichia coli is one of the most main food borne pathogen causing serious problem particular for human through food of animal origin such as meat and dairy products. The aim of this study was to determine the prevalence, antibiogram pattern and molecular identification of some putative virulence genes of pathogenic E.coli recovered from human and food sources. A total of 200 samples from minced meat, (50) Karish cheese, (50) human stool sample (50) and human urine samples (50) were collected for bacteriological identification before being subjected to serological and molecular analysis. The results revealed that 123 (61.5%) were positive for E. coli species on MacConkey ager while only 35 isolates were give green metallic sheen colonies on EMB. Of these 35 isolates, 16 isolates (45.7%) were identified as pathogenic E.coli through biofilm activity on congo red medium (back color colonies). The serological identification of 16 these E.coli strains revealed that EHEC was the most prevalent serotype and found in 8 (50%) followed by EPEC and ETEC 4(25%) for each serotype. All isolates showed high sensitivity test and found that amikacin, nitrofurantoin, gentamicin, amoxicillin /clavulanic acid, ciprofloxacin, and Ceftriaxone exhibit highly sensitivity with 100, 100, 87.5, 75, 62.5, and 62.5% respectively while resistance was recorded for penicillin, tetracycline, Trimethoprim-Sulfamethoxazole and colistin with 75, 75, 50% and 50% respectively. The PCR assay was applied to identify fimA, eaeA, and sxt1virulence genes by using specific primers sets. The results reported that out of screening 10 stains , fimA gene was found in all examined strains (100%), while the eaeA gene was detected in 5 (50%) with no detection of sxt1 gene. In conclusion, the obtained results provide an important data about the relation between human disease and consumption of meat and milk products and understand the epidemiology and disease occurrence.

Key words: Meat, Cheese, pathogenic E.coli, Virulence, Antibiogram


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Eman M. Omr and Eman E. Abdeen
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Eman M. Omr and Eman E. Abdeen. Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. AJVS. 2023; 78(1): 9-16. doi:10.5455/ajvs.155327


Web Style

Eman M. Omr and Eman E. Abdeen. Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. https://www.alexjvs.com/?mno=155327 [Access: May 04, 2025]. doi:10.5455/ajvs.155327


AMA (American Medical Association) Style

Eman M. Omr and Eman E. Abdeen. Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. AJVS. 2023; 78(1): 9-16. doi:10.5455/ajvs.155327



Vancouver/ICMJE Style

Eman M. Omr and Eman E. Abdeen. Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. AJVS. (2023), [cited May 04, 2025]; 78(1): 9-16. doi:10.5455/ajvs.155327



Harvard Style

Eman M. Omr and Eman E. Abdeen (2023) Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. AJVS, 78 (1), 9-16. doi:10.5455/ajvs.155327



Turabian Style

Eman M. Omr and Eman E. Abdeen. 2023. Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. Alexandria Journal of Veterinary Sciences, 78 (1), 9-16. doi:10.5455/ajvs.155327



Chicago Style

Eman M. Omr and Eman E. Abdeen. "Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources." Alexandria Journal of Veterinary Sciences 78 (2023), 9-16. doi:10.5455/ajvs.155327



MLA (The Modern Language Association) Style

Eman M. Omr and Eman E. Abdeen. "Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources." Alexandria Journal of Veterinary Sciences 78.1 (2023), 9-16. Print. doi:10.5455/ajvs.155327



APA (American Psychological Association) Style

Eman M. Omr and Eman E. Abdeen (2023) Prevalence, Antibiogram Pattern and Molecular Identification of pathogenic E. coli recovered from Different Sources. Alexandria Journal of Veterinary Sciences, 78 (1), 9-16. doi:10.5455/ajvs.155327





Most Viewed Articles
Most Downloaded
Most Cited Articles