Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica

Shimaa A. Khatab; Shabaan A. Hemeda; Abeer F. El-Nahas; Walaa S. Abd El Naby

doi:10.5455/ajvs.167827

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Original Article
Online Published: 08 Oct 2014

AJVS. 2014; 43(1): 26-32

doi: 10.5455/ajvs.167827

Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica

Shimaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-nahas, Walaa S. Abd El Naby.

Abstract
Insulin-like growth factor II (IGF-II) is a crucial factor in regulating cell proliferation, growth, migration, differentiation and survival. The objective of this study was to investigate the association of polymorphism in intron 3 and exon 3 of insulin like growth factor II (IGF-II) gene and body weight trait in tilapia nilotica fish using restriction fragment length polymorphism (RFLP) and single nucleotide polymorphism (SNP) techniques. Thirty tilapia nilotica fish were precisely selected according to their body weight and ordered from the largest to the smallest body size. DNA was extracted from fish blood samples to amplify 397-bp of IGF-II gene. Amplified IGF-II gene was sequenced only in the seven large and five small size fish. Results revealed that restriction analysis (RFLP) with MSP1 of the (IGF-II) gene (397-bp) do not produce restriction fragments. While, DNA sequencing showed fourteen single nucleotide polymorphisms (SNPs) at different positions in large size fish as they were detected in more than one fish at nucleotides number 318 (C→T), 319 (A→T), 320 (G→A in three fish), 322 (C→A and C→G), 323 (G→C and G→A), 326 (G→A in three fish), 332 (A→T in three fish), 333 (C→A and C→T), 334 (A→C in two fish), 337 (C→T in two fish) and 340 (C→T). Hence, our results reveal the possibility to use these findings as marker for selection of high body weight trait in tilapia nilotica fish.

Key words: Tilapia Nilotica, Insulin like growth factor- II, Single Nucleotide Polymorphism

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How to Cite this Article

Pubmed Style

Khatab SA, Hemeda SA, El-nahas AF, Naby WSAE. Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. AJVS. 2014; 43(1): 26-32. doi:10.5455/ajvs.167827

Web Style

Khatab SA, Hemeda SA, El-nahas AF, Naby WSAE. Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. https://www.alexjvs.com/?mno=167827 [Access: May 03, 2025]. doi:10.5455/ajvs.167827

AMA (American Medical Association) Style

Khatab SA, Hemeda SA, El-nahas AF, Naby WSAE. Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. AJVS. 2014; 43(1): 26-32. doi:10.5455/ajvs.167827

Vancouver/ICMJE Style

Khatab SA, Hemeda SA, El-nahas AF, Naby WSAE. Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. AJVS. (2014), [cited May 03, 2025]; 43(1): 26-32. doi:10.5455/ajvs.167827

Harvard Style

Khatab, S. A., Hemeda, . S. A., El-nahas, . A. F. & Naby, . W. S. A. E. (2014) Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. AJVS, 43 (1), 26-32. doi:10.5455/ajvs.167827

Turabian Style

Khatab, Shimaa A., Shabaan A. Hemeda, Abeer F. El-nahas, and Walaa S. Abd El Naby. 2014. Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. Alexandria Journal of Veterinary Sciences, 43 (1), 26-32. doi:10.5455/ajvs.167827

Chicago Style

Khatab, Shimaa A., Shabaan A. Hemeda, Abeer F. El-nahas, and Walaa S. Abd El Naby. "Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica." Alexandria Journal of Veterinary Sciences 43 (2014), 26-32. doi:10.5455/ajvs.167827

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Khatab, S. A., Hemeda, . S. A., El-nahas, . A. F. & Naby, . W. S. A. E. (2014) Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica. Alexandria Journal of Veterinary Sciences, 43 (1), 26-32. doi:10.5455/ajvs.167827

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