E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
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Original Article
Online Published: 25 Jan 2016
 


Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits

Eslam A. Asran, Samy A Khalil, Abd El-halim M. Hegazy.


Abstract
A total of 117 samples were collected from individual cases of diseased rabbits showed respiratory signs (68 samples from nasopharyngeal swabs, 43 samples from lung tissues, 2 samples from subcutaneous abscesses swabs, 3 samples from liver swabs and 1 swab from the conjunctiva). During this study 11 Pasteurella multocida isolates were isolated with percentage of 9.4% as 7 isolates from nasal swabs (6%) and 4 isolates from lung tissues (3.4%). The samples examined phenotypically (morphology and biochemical characters of Pasteurella multocida) and tested by PCR technique for confirmatory identification and detected the presence of capsule, hemagglutinin and type IV fimbria genes as virulence factors. This study confirmed the presence of capsule in 100% of the examined samples (6/6) and hemagglutinin in 83.3% (5/6) examined samples and type IV fimbria in 33.3% (2/6) examined samples. It was concluded that all the examined Pasteurella multocida samples in this study were virulent and the multiplex-PCR protocols could be useful tools for rapid and simultaneous detection of Pasteurella multocida virulence genes.

Key words: Pasteurella multocida, virulence factors, capsule, hemagglutinin, type IV fimbria multiplex PCR.


 
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How to Cite this Article
Pubmed Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS. 2016; 48(1): 34-41. doi:10.5455/ajvs.187808

Web Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. https://www.alexjvs.com/?mno=187808 [Access: May 06, 2025]. doi:10.5455/ajvs.187808

AMA (American Medical Association) Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS. 2016; 48(1): 34-41. doi:10.5455/ajvs.187808


Vancouver/ICMJE Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS. (2016), [cited May 06, 2025]; 48(1): 34-41. doi:10.5455/ajvs.187808


Harvard Style

Asran, E. A., Khalil, . S. A. & Hegazy, . A. E. M. (2016) Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS, 48 (1), 34-41. doi:10.5455/ajvs.187808


Turabian Style

Asran, Eslam A., Samy A Khalil, and Abd El-halim M. Hegazy. 2016. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. Alexandria Journal of Veterinary Sciences, 48 (1), 34-41. doi:10.5455/ajvs.187808


Chicago Style

Asran, Eslam A., Samy A Khalil, and Abd El-halim M. Hegazy. "Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits." Alexandria Journal of Veterinary Sciences 48 (2016), 34-41. doi:10.5455/ajvs.187808


MLA (The Modern Language Association) Style

Asran, Eslam A., Samy A Khalil, and Abd El-halim M. Hegazy. "Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits." Alexandria Journal of Veterinary Sciences 48.1 (2016), 34-41. Print. doi:10.5455/ajvs.187808


APA (American Psychological Association) Style

Asran, E. A., Khalil, . S. A. & Hegazy, . A. E. M. (2016) Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. Alexandria Journal of Veterinary Sciences, 48 (1), 34-41. doi:10.5455/ajvs.187808




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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