Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits

Eslam A. Asran; Samy A Khalil; Abd El-Halim M. Hegazy

doi:10.5455/ajvs.187808

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Original Article
Online Published: 25 Jan 2016

AJVS. 2016; 48(1): 34-41

doi: 10.5455/ajvs.187808

Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits

Eslam A. Asran, Samy A Khalil, Abd El-halim M. Hegazy.

Abstract
A total of 117 samples were collected from individual cases of diseased rabbits showed respiratory signs (68 samples from nasopharyngeal swabs, 43 samples from lung tissues, 2 samples from subcutaneous abscesses swabs, 3 samples from liver swabs and 1 swab from the conjunctiva). During this study 11 Pasteurella multocida isolates were isolated with percentage of 9.4% as 7 isolates from nasal swabs (6%) and 4 isolates from lung tissues (3.4%). The samples examined phenotypically (morphology and biochemical characters of Pasteurella multocida) and tested by PCR technique for confirmatory identification and detected the presence of capsule, hemagglutinin and type IV fimbria genes as virulence factors. This study confirmed the presence of capsule in 100% of the examined samples (6/6) and hemagglutinin in 83.3% (5/6) examined samples and type IV fimbria in 33.3% (2/6) examined samples. It was concluded that all the examined Pasteurella multocida samples in this study were virulent and the multiplex-PCR protocols could be useful tools for rapid and simultaneous detection of Pasteurella multocida virulence genes.

Key words: Pasteurella multocida, virulence factors, capsule, hemagglutinin, type IV fimbria multiplex PCR.

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Pubmed Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS. 2016; 48(1): 34-41. doi:10.5455/ajvs.187808

Web Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. https://www.alexjvs.com/?mno=187808 [Access: May 06, 2025]. doi:10.5455/ajvs.187808

AMA (American Medical Association) Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS. 2016; 48(1): 34-41. doi:10.5455/ajvs.187808

Vancouver/ICMJE Style

Asran EA, Khalil SA, Hegazy AEM. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS. (2016), [cited May 06, 2025]; 48(1): 34-41. doi:10.5455/ajvs.187808

Harvard Style

Asran, E. A., Khalil, . S. A. & Hegazy, . A. E. M. (2016) Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. AJVS, 48 (1), 34-41. doi:10.5455/ajvs.187808

Turabian Style

Asran, Eslam A., Samy A Khalil, and Abd El-halim M. Hegazy. 2016. Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. Alexandria Journal of Veterinary Sciences, 48 (1), 34-41. doi:10.5455/ajvs.187808

Chicago Style

Asran, Eslam A., Samy A Khalil, and Abd El-halim M. Hegazy. "Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits." Alexandria Journal of Veterinary Sciences 48 (2016), 34-41. doi:10.5455/ajvs.187808

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Asran, E. A., Khalil, . S. A. & Hegazy, . A. E. M. (2016) Identification and Molecular Analysis of Pasteurella Multocida Isolated from Rabbits. Alexandria Journal of Veterinary Sciences, 48 (1), 34-41. doi:10.5455/ajvs.187808

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