Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples

Alaa Eldin H. Mustapha; Maha M. Mansour

doi:10.5455/ajvs.196375

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Original Article

AJVS. 2024; 81(0): 38-50

doi: 10.5455/ajvs.196375

Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples

Alaa Eldin H. Mustapha, Maha M. Mansour.

Abstract
A total of 260 samples of raw milk were gathered from a variety of farms, street vendors, residences, and dairy stores in the governorate of EL-Menoufia. 220 (84.6%) of the 260 samples had Enterobacteriaceae. At species level, Klebsiella and E. coli were most predominant at 40.4% and 32.6% respectively, followed by 21.2% Citrobacter freundii and 7.3% Proteus while Salmonella showed the lowest 1.9%. Using SYBER green real-time PCR, thirty uncultured samples were subjected to rpoB genotypic identification for viable Enterobacteriaceae detection, and all thirty (100%) of the samples showed the gene. By using SYBER green real-time PCR after PMA treatment, 10 (33.3%) of these 30 culture-negative samples showed the presence of VBNC enterobacterial isolates.

Key words: Propidium Monoazide PCR, real-time PCR, and viable but non-cultivable Enterobacteriaceae

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Pubmed Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS. 2024; 81(0): 38-50. doi:10.5455/ajvs.196375

Web Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. https://www.alexjvs.com/?mno=196375 [Access: May 06, 2025]. doi:10.5455/ajvs.196375

AMA (American Medical Association) Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS. 2024; 81(0): 38-50. doi:10.5455/ajvs.196375

Vancouver/ICMJE Style

Mustapha AEH, Mansour MM. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS. (2024), [cited May 06, 2025]; 81(0): 38-50. doi:10.5455/ajvs.196375

Harvard Style

Mustapha, A. E. H. & Mansour, . M. M. (2024) Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. AJVS, 81 (0), 38-50. doi:10.5455/ajvs.196375

Turabian Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. 2024. Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples. Alexandria Journal of Veterinary Sciences, 81 (0), 38-50. doi:10.5455/ajvs.196375

Chicago Style

Mustapha, Alaa Eldin H., and Maha M. Mansour. "Using of Real Time PCR with Propidium Mono Azide for Detection of Viable but Non Culturable Enterobacteriaceae Isolated from Raw Milk Samples." Alexandria Journal of Veterinary Sciences 81 (2024), 38-50. doi:10.5455/ajvs.196375

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APA (American Psychological Association) Style

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