Abstract
Arthritis in broiler chickens is one of economic problems causing losses in poultry industry. Hundred samples (60 samples for Mycoplasma synoviae isolation and 40 samples for isolation other bacteria) from chicken with arthritis symptoms, were collected from different broiler chickens farms. The samples were cultivated on PPIO media for isolation of Mycoplasma synoviae and different media( MaCconky , EMB, X.L.D and salt Mannitol media) for isolation of other bacteria and biochemically identified. The results revealed that out of 40 samples 28(70%) were positive for E. coli isolation,10 (25%) were positive for Staphylococcus aureus isolation,2(5%) were positive for Salmonella Enterica isolation. Out of 6osamples 6(10%) were positive for Mycoplasma synoviae isolation. Amplification of vlhA gene of Mycoplasma synoviae showed that out of 6 isolate 3 (50%) were positive for the gene . Six seogroups of E. coli isolates examined serologically and put into six group, one each group (O128, O125, O146,O27,O114 and O158).The result of multiplex PCR for E.coli virulence genes( ibeA and iss ) showed that iss were detected in all serogroups .While ibeA virulence gene was detected in serotypes O125 and O146 only .The result of PCR for amplification of virulance genes of S.aureus(CNA,clfA)reveled that out of 8 isolate of S.aureus,3(37.5%) were positive for clfA. Out of 3 positive S.aureus for clfA,3(100%) were positive for CNA.

Key words: Mycoplasma synoviae, other associated bacteria ,Virulance genes , PCR ,Arthritis and Chickens