Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa

Aly A. El-Sharkawy; Sayed A Hattab; Ismail I. Abo-Ghaniema; El-Garhy N El-Garhy; Magdy kh Soliman

2025, Vol: 87

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Original Article

AJVS. 0; 0(0):

Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa

Aly A. El-sharkawy, Sayed A Hattab, Ismail I. Abo-ghaniema, El-garhy N El-garhy, Magdy Kh Soliman.

Abstract
This study aimed to compare the efficiency of four cryodiluents on freezability and mitochondrial activity of Arab stallion spermatozoa. Semen samples were collected from 10 Arab stallions, diluted with modified INRA-82 (m-INRA), modified Kenneys (m-Kenney) and or Eqi-Pro® diluents and subjected to cryopreservation. Post-thaw motility, membrane, acrosomal and mitochondrial integrities were evaluated. The results revealed the superiority of m-INRA over m-Kenney and or Eqi-Pro® in terms of post-thaw motility (52.50 ± 2.27 vs. 43.00 ± 2.13 and 41.50 ± 2.59%, respectively), viability index (134.25 ± 5.99 vs. 97.50 ± 6.40 and 94.75 ± 7.33, respectively), sperm membrane (46.60 ± 2.05 vs. 36.70 ± 1.73 and 36.60 ± 2.09%, respectively) and acrosome integrities (54.50 ± 1.30 vs. 43.00 ± 1.86 and 43.5 ±1.86%). Activities of stallion sperm mitochondria were better preserved using m-INRA as cryodiluent. Using Kenneys extender resulted in the lowest sperm motility and integrities of acrosome, membranes and mitochondria. In conclusion, the motility as well as functional integrities of Arab stallion spermatozoa could be enhanced using m-INRA as a cryodiluent.

Key words: Extenders, mitochondrial activity, Arab stallion, spermatozoa

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Pubmed Style

El-sharkawy AA, Hattab SA, Abo-ghaniema II, El-garhy EN, Soliman MK. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS. 0; 0(0): -.

Web Style

El-sharkawy AA, Hattab SA, Abo-ghaniema II, El-garhy EN, Soliman MK. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. https://www.alexjvs.com/?mno=240986 [Access: May 03, 2025].

AMA (American Medical Association) Style

El-sharkawy AA, Hattab SA, Abo-ghaniema II, El-garhy EN, Soliman MK. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS. 0; 0(0): -.

Vancouver/ICMJE Style

El-sharkawy AA, Hattab SA, Abo-ghaniema II, El-garhy EN, Soliman MK. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS. (0), [cited May 03, 2025]; 0(0): -.

Harvard Style

El-sharkawy, A. A., Hattab, . S. A., Abo-ghaniema, . I. I., El-garhy, . E. N. & Soliman, . M. K. (0) Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS, 0 (0), -.

Turabian Style

El-sharkawy, Aly A., Sayed A Hattab, Ismail I. Abo-ghaniema, El-garhy N El-garhy, and Magdy Kh Soliman. 0. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. Alexandria Journal of Veterinary Sciences, 0 (0), -.

Chicago Style

El-sharkawy, Aly A., Sayed A Hattab, Ismail I. Abo-ghaniema, El-garhy N El-garhy, and Magdy Kh Soliman. "Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa." Alexandria Journal of Veterinary Sciences 0 (0), -.

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