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Original Article 


Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels

Emad N. Hegazy, Abd El-kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, Amir H. Abd El-rahman.


Abstract
This study was designed to evaluate PCR technique as a diagnostic tool for blood parasites infection in equine and camels in relation to conventional microscopic examination of stained blood smear. 150 equine stained blood smears were examined microscopically, 15 of them were suspected to be infested with equine piroplasmosis. 10 samples (including one positive by blood film) were examined by conventional PCR technique, 7 samples were positive. 135 camels stained blood smears were examined microscopically, 2 of them were infested with trypanosoma, and 10, 5 and 15 samples were suspected to be infested with theileria spp., babesia spp. and anaplasma spp., respectively. 10 samples (including 2 positive for trypanosoma), 10 negative for theileria, 10 negative for babesia and 10 negative for anaplasma by blood film were examined separately by conventional PCR technique, 9, 1, 1 and 5 samples were positive, respectively. The results concluded that PCR assays are more sensitive than blood film examination.

Key words: PCR, blood smear, equine, camels, piroplasmois, Trypansoma, Theileria, Babesia, Anaplasma


 
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How to Cite this Article
Pubmed Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS. 2017; 55(1): 217-224. doi:10.5455/ajvs.261935

Web Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. https://www.alexjvs.com/?mno=261935 [Access: May 05, 2025]. doi:10.5455/ajvs.261935

AMA (American Medical Association) Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS. 2017; 55(1): 217-224. doi:10.5455/ajvs.261935


Vancouver/ICMJE Style

Hegazy EN, Mahmoud AEA, Khadr AM, Elshemey TM, El-rahman AHA. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS. (2017), [cited May 05, 2025]; 55(1): 217-224. doi:10.5455/ajvs.261935


Harvard Style

Hegazy, E. N., Mahmoud, . A. E. A., Khadr, . A. M., Elshemey, . T. M. & El-rahman, . A. H. A. (2017) Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. AJVS, 55 (1), 217-224. doi:10.5455/ajvs.261935


Turabian Style

Hegazy, Emad N., Abd El-kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, and Amir H. Abd El-rahman. 2017. Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. Alexandria Journal of Veterinary Sciences, 55 (1), 217-224. doi:10.5455/ajvs.261935


Chicago Style

Hegazy, Emad N., Abd El-kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, and Amir H. Abd El-rahman. "Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels." Alexandria Journal of Veterinary Sciences 55 (2017), 217-224. doi:10.5455/ajvs.261935


MLA (The Modern Language Association) Style

Hegazy, Emad N., Abd El-kareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, and Amir H. Abd El-rahman. "Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels." Alexandria Journal of Veterinary Sciences 55.1 (2017), 217-224. Print. doi:10.5455/ajvs.261935


APA (American Psychological Association) Style

Hegazy, E. N., Mahmoud, . A. E. A., Khadr, . A. M., Elshemey, . T. M. & El-rahman, . A. H. A. (2017) Evaluation of Molecular Technique and Microscopical Examination for Diagnosis of Blood Parasites In Equine and Camels. Alexandria Journal of Veterinary Sciences, 55 (1), 217-224. doi:10.5455/ajvs.261935




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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