E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources

Eman H. Abotalp, Eman Abdeen, Alaa El-din H. Moustafa, Sahar R. Mohamed.


Abstract
Escherichia coli O157 is recognized as an important foodborne pathogen responsible for sporadic cases to serious outbreaks worldwide. The morbidity and mortality associated with several recent outbreaks due to STEC have highlighted the threat this organism possess to public health. Bacteriological examination of (671) samples collected from cattle, sheep, chickens, ducks, milk and water showed that 34.3% (230/671) were positive E. coli. Out of (230) isolates of positive E. coli, (16) isolate were identified by vitek2 system as E. coli O157 (6.9%) with high incidence in cattle samples (20%) then chicken and water samples (13.6%) and (12.5%) respectively. On the other hand, there were no isolates from ducks samples for E. coli O157 and with low percent (7.8%) from raw milk samples and (1%) from sheep samples. The application of PCR for screening of virulence genes (sxt1, sxt2 and eaeA) in E. coli O157 isolates revealed that out of (16) isolates of E. coli O157 there were (9), (6) and (16) were positive for stx1, stx2 and eaeA, respectively. This study concluded that molecular identification by PCR for detection of E. coli O157 virulence genes is rapid, specific and accurate approach in studying the actual role of these genes in disease pathogenicity.

Key words: E.coli O157, virulence factor and sequence of eaeA gene).


 
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How to Cite this Article
Pubmed Style

Abotalp EH, Abdeen E, Moustafa AEH, Mohamed SR. Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. AJVS. 2017; 53(2): 38-44. doi:10.5455/ajvs.262860

Web Style

Abotalp EH, Abdeen E, Moustafa AEH, Mohamed SR. Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. https://www.alexjvs.com/?mno=262860 [Access: May 06, 2025]. doi:10.5455/ajvs.262860

AMA (American Medical Association) Style

Abotalp EH, Abdeen E, Moustafa AEH, Mohamed SR. Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. AJVS. 2017; 53(2): 38-44. doi:10.5455/ajvs.262860


Vancouver/ICMJE Style

Abotalp EH, Abdeen E, Moustafa AEH, Mohamed SR. Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. AJVS. (2017), [cited May 06, 2025]; 53(2): 38-44. doi:10.5455/ajvs.262860


Harvard Style

Abotalp, E. H., Abdeen, . E., Moustafa, . A. E. H. & Mohamed, . S. R. (2017) Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. AJVS, 53 (2), 38-44. doi:10.5455/ajvs.262860


Turabian Style

Abotalp, Eman H., Eman Abdeen, Alaa El-din H. Moustafa, and Sahar R. Mohamed. 2017. Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 53 (2), 38-44. doi:10.5455/ajvs.262860


Chicago Style

Abotalp, Eman H., Eman Abdeen, Alaa El-din H. Moustafa, and Sahar R. Mohamed. "Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 53 (2017), 38-44. doi:10.5455/ajvs.262860


MLA (The Modern Language Association) Style

Abotalp, Eman H., Eman Abdeen, Alaa El-din H. Moustafa, and Sahar R. Mohamed. "Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 53.2 (2017), 38-44. Print. doi:10.5455/ajvs.262860


APA (American Psychological Association) Style

Abotalp, E. H., Abdeen, . E., Moustafa, . A. E. H. & Mohamed, . S. R. (2017) Molecular Detection of Virulence Genes of Escherichia Coli O157 Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 53 (2), 38-44. doi:10.5455/ajvs.262860




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    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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