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Original Article 


Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa

Ahmed M. El-ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel.


Abstract
In vitro fertilization (IVF) is a necessary procedure in the in vitro production of embryo. Spermatozoa need to undergo capacitation to be able to perform the acrosome reaction which is required for zona pellucida penetration. Different additives are used to aid in that process, such as caffeine and theophylline. In this study, the effects of IVF media supplementation with D- penicillamine, Hypotaurine and epinephrine (PHE) on fertilization and cleavage rates were assessed. Cumulus oocyte complexes (COCs) containing a compact cumulus cells and normal homogenous cytoplasm were selected. In vitro maturation (IVM) was performed in TCM199 supplied with hormones overnight in a culture conditions of 38.5°C and 5% CO2 in air. After 20-22 hr. of maturation, oocytes were randomly assigned to five groups of sperm-TALP media (control and four different PHE concentrations) then inseminated. The fertilization rate of the expected embryos were assessed after 22 hr. after staining with aceto-orcin 1% or cultured for 48 hr. to estimate the cleavage rate. The current data revealed that, 25 µl/ml PHE significantly (P< 0.05) improved fertilization (55.93 vs 36.96%) and cleavage (46.15 vs 22.22%) rates when compared to control.

Key words: D- Penecillamine, Hypotaurine, Epinephrine, In vitro fertilization, Buffalo


 
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How to Cite this Article
Pubmed Style

El-ruby AM, Hussein MS, Bedier W, Montaser AM, Badr MR, Hegab AO, Zaabel SM. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS. 2017; 53(2): 6-10. doi:10.5455/ajvs.264914

Web Style

El-ruby AM, Hussein MS, Bedier W, Montaser AM, Badr MR, Hegab AO, Zaabel SM. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. https://www.alexjvs.com/?mno=264914 [Access: May 03, 2025]. doi:10.5455/ajvs.264914

AMA (American Medical Association) Style

El-ruby AM, Hussein MS, Bedier W, Montaser AM, Badr MR, Hegab AO, Zaabel SM. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS. 2017; 53(2): 6-10. doi:10.5455/ajvs.264914


Vancouver/ICMJE Style

El-ruby AM, Hussein MS, Bedier W, Montaser AM, Badr MR, Hegab AO, Zaabel SM. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS. (2017), [cited May 03, 2025]; 53(2): 6-10. doi:10.5455/ajvs.264914


Harvard Style

El-ruby, A. M., Hussein, . M. S., Bedier, . W., Montaser, . A. M., Badr, . M. R., Hegab, . A. O. & Zaabel, . S. M. (2017) Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS, 53 (2), 6-10. doi:10.5455/ajvs.264914


Turabian Style

El-ruby, Ahmed M., Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, and Samy M. Zaabel. 2017. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. Alexandria Journal of Veterinary Sciences, 53 (2), 6-10. doi:10.5455/ajvs.264914


Chicago Style

El-ruby, Ahmed M., Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, and Samy M. Zaabel. "Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa." Alexandria Journal of Veterinary Sciences 53 (2017), 6-10. doi:10.5455/ajvs.264914


MLA (The Modern Language Association) Style

El-ruby, Ahmed M., Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, and Samy M. Zaabel. "Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa." Alexandria Journal of Veterinary Sciences 53.2 (2017), 6-10. Print. doi:10.5455/ajvs.264914


APA (American Psychological Association) Style

El-ruby, A. M., Hussein, . M. S., Bedier, . W., Montaser, . A. M., Badr, . M. R., Hegab, . A. O. & Zaabel, . S. M. (2017) Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. Alexandria Journal of Veterinary Sciences, 53 (2), 6-10. doi:10.5455/ajvs.264914




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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