E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli

Samar A. Swelam, Abbas A. Amin, Amer A. Amer, Hussin S. Abo El-makarem.


Abstract
Milk as it comes from a normal udder of a healthy animal contains a negligible number of harmful organisms but may be contaminated from variable sources. Forty random samples of raw milk were collected from supermarkets in Alexandria governorate for isolation and identification of some pathogenic microorganisms using conventional methods and advanced techniques. incidence of E. coli, Staph.aureus, listeria monocytogens and salmonella were 80, 45, 5 and 0%, respectively by using plating techniques. These results were confirmed by conventional biochemical identifications but by using vitek 2 compact system (automated biochemical identifications), the incidenceswere 37.5, 2.5, 0 and 0%, respectively.The incidence of Shiga toxin-producing E. coli isolated from the examined raw milk samples (4/10%) and could be identify virulence genes intofour different serotypes including O26:H11 (EHEC), O86 (EHEC), O119:H6 (ETEC) and O125:H21 (EHEC). By using multiplex PCR, it was found that O26:H11 had shigatoxin 1 gene, shigatoxin 2 gene and intimin gene; while O86 and O119:H6 serotypes had only shigatoxin1 gene. O125:H21 serotype had both shigatoxin 1 and shigatoxin 2 genes. It could be concluded that vitek 2 compact system is considered as sensitive and accurate method for identification of some food poisoning microorganisms in comparing with conventional biochemical identifications.

Key words: E. coli, Staph.aureus, listeria monocytogenes, raw milk, viteck 2 compact


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Samar A. Swelam
Articles by Abbas A. Amin
Articles by Amer A. Amer
Articles by Hussin S. Abo El-Makarem
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. 2017; 54(2): 99-105. doi:10.5455/ajvs.267275

Web Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. https://www.alexjvs.com/?mno=267275 [Access: May 04, 2025]. doi:10.5455/ajvs.267275

AMA (American Medical Association) Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. 2017; 54(2): 99-105. doi:10.5455/ajvs.267275


Vancouver/ICMJE Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. (2017), [cited May 04, 2025]; 54(2): 99-105. doi:10.5455/ajvs.267275


Harvard Style

Swelam, S. A., Amin, . A. A., Amer, . A. A. & El-makarem, . H. S. A. (2017) Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS, 54 (2), 99-105. doi:10.5455/ajvs.267275


Turabian Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-makarem. 2017. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. Alexandria Journal of Veterinary Sciences, 54 (2), 99-105. doi:10.5455/ajvs.267275


Chicago Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-makarem. "Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli." Alexandria Journal of Veterinary Sciences 54 (2017), 99-105. doi:10.5455/ajvs.267275


MLA (The Modern Language Association) Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-makarem. "Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli." Alexandria Journal of Veterinary Sciences 54.2 (2017), 99-105. Print. doi:10.5455/ajvs.267275


APA (American Psychological Association) Style

Swelam, S. A., Amin, . A. A., Amer, . A. A. & El-makarem, . H. S. A. (2017) Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. Alexandria Journal of Veterinary Sciences, 54 (2), 99-105. doi:10.5455/ajvs.267275




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    Author LoginReviewer LoginAbout PublisherEditorial PoliciesEditorial Review PolicyPeer Review PolicyEditorial & Peer Review ProcessAuthor's Rights and ObligationsPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information