In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus

Hatem S. Abd El-Hamid; Hany F. Ellakany; Ahmed R. Elbestawy; Abdalla A. Seliem; Hazem S. Khalil

doi:10.5455/ajvs.281430

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Original Article

AJVS. 2018; 56(1): 145-152

doi: 10.5455/ajvs.281430

In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus

Hatem S. Abd El-hamid, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, Hazem S. Khalil.

Abstract
Three commercial products of herbal extracts (Amphiflu-Ex®, Fyto-immune Plus® and Viu-Vet®) were tested for antiviral activity against two Egyptian highly pathogenic avian influenza subtype H5N1 isolates (isolated form backyard poultry) and upon sequencing and phylogenetic analysis, they belonged to clade 2.2.1.2., This antiviral assay for the products done by using chicken embryo fibroblast (CEF) cell culture through cytopathic effect (CPE) inhibition assay in two different layouts to elucidate post-exposure and pre-exposure antiviral activity of the products. The Amphiflu-Ex® at conc. 50 µl/ml showed significant virucidal activity (100% inhibition) in post-exposure assay for both viral isolates which was further confirmed by quantitative real time RT-PCR method showed 100% reduction in virus titers for both isolates and further investigation done on The Amphiflu-Ex® on conc. 20 µl/ml, the virus titers were decreased by 2.72-2.8 logs (Log 10 virus titers) and on conc 10 µl/ml, the virus titers were decreased by 1-1.27 logs for both isolates, The maximum non-toxic concentration (MNTC) for the three products estimated by cytotoxicity assay was 50 µl/ml and TCID50 used for both isolates were 106 copy/100 µl.

Key words: Avian influenza virus subtype- H5N1, Antiviral activity, Herbal extracts, RT-PCR.

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How to Cite this Article

Pubmed Style

El-hamid HSA, Ellakany HF, Elbestawy AR, Seliem AA, Khalil HS. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS. 2018; 56(1): 145-152. doi:10.5455/ajvs.281430

Web Style

El-hamid HSA, Ellakany HF, Elbestawy AR, Seliem AA, Khalil HS. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. https://www.alexjvs.com/?mno=281430 [Access: May 05, 2025]. doi:10.5455/ajvs.281430

AMA (American Medical Association) Style

El-hamid HSA, Ellakany HF, Elbestawy AR, Seliem AA, Khalil HS. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS. 2018; 56(1): 145-152. doi:10.5455/ajvs.281430

Vancouver/ICMJE Style

El-hamid HSA, Ellakany HF, Elbestawy AR, Seliem AA, Khalil HS. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS. (2018), [cited May 05, 2025]; 56(1): 145-152. doi:10.5455/ajvs.281430

Harvard Style

El-hamid, H. S. A., Ellakany, . H. F., Elbestawy, . A. R., Seliem, . A. A. & Khalil, . H. S. (2018) In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. AJVS, 56 (1), 145-152. doi:10.5455/ajvs.281430

Turabian Style

El-hamid, Hatem S. Abd, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, and Hazem S. Khalil. 2018. In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus. Alexandria Journal of Veterinary Sciences, 56 (1), 145-152. doi:10.5455/ajvs.281430

Chicago Style

El-hamid, Hatem S. Abd, Hany F. Ellakany, Ahmed R. Elbestawy, Abdalla A. Seliem, and Hazem S. Khalil. "In Vitro Antiviral Activity of Commercial Products of Herbal Extracts Against Highly Pathogenic Avian Influenza (H5N1) Virus." Alexandria Journal of Veterinary Sciences 56 (2018), 145-152. doi:10.5455/ajvs.281430

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APA (American Psychological Association) Style

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