E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 80, Issue: 1
80 / 1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey.

Abstract
Proliferating cell nuclear antigen (PCNA) coordinates many functions including cell cycle progression and DNA replication in eukaryotic cells. Inhibition of the PCNA protein function in Trypanosoma brucei could be a new strategy in controlling Human African Trypanosomiasis. In the present study, we explored the function of TbPCNA by deregulation the protein expression through the overexpression, and by applying the small molecule inhibition. Overexpression of human PCNA or T. brucei PCNA wild-type proteins inhibits the parasite proliferation. On the other hand, T. brucei that overexpress the HsPCNA M40A or TbPCNA M40A mutants grew normally, indicating that inhibition of PIP-box protein interaction is important for the lethal phenotype. In testing this hypothesis, we applied the small molecule inhibitor T2 amino alcohol (T2AA). Proliferation in parasites that overexpress HsPCNA resumed after T2AA treatment, but not parasites that overexpressed TbPCNA. We concluded that T2AA selectively inhibits HsPCNA not TbPCNA. This data presented here will be a prerequisite for investigating TbPCNA interacting proteins and discovering inhibitors that specifically target TbPCNA to kill the parasite.

Key words: T2 amino alcohol, proliferating cell nuclear antigen and Trypanosoma brucei.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Yasser N. Haggag
Articles by Mohammad A. Nossair
Articles by Haitham Elaadli
Articles by Zachary Mackey
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. AJVS. 2018; 56(1): 81-87. doi:10.5455/ajvs.285213

Web Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. https://www.alexjvs.com/?mno=285213 [Access: December 16, 2023]. doi:10.5455/ajvs.285213

AMA (American Medical Association) Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. AJVS. 2018; 56(1): 81-87. doi:10.5455/ajvs.285213


Vancouver/ICMJE Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. AJVS. (2018), [cited December 16, 2023]; 56(1): 81-87. doi:10.5455/ajvs.285213


Harvard Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey (2018) Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. AJVS, 56 (1), 81-87. doi:10.5455/ajvs.285213


Turabian Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. 2018. Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. Alexandria Journal of Veterinary Sciences, 56 (1), 81-87. doi:10.5455/ajvs.285213


Chicago Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. "Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei." Alexandria Journal of Veterinary Sciences 56 (2018), 81-87. doi:10.5455/ajvs.285213


MLA (The Modern Language Association) Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey. "Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei." Alexandria Journal of Veterinary Sciences 56.1 (2018), 81-87. Print. doi:10.5455/ajvs.285213


APA (American Psychological Association) Style

Yasser N. Haggag, Mohammad A. Nossair, Haitham Elaadli, Zachary Mackey (2018) Selective inhibition of T2AA for human PCNA over PCNA homolog in Trypanosoma brucei. Alexandria Journal of Veterinary Sciences, 56 (1), 81-87. doi:10.5455/ajvs.285213




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine, Alexandria University
    Edfina, Behaira, Egypt.
    PO.Box: 22758
    Telefax: +20 45 2963526
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use