Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle

Hazem Farouk Mahmoud; Gaber S. Abdellrazeq; Mahmoud M. El-Naggar; Essam Amin Nasr; Samy Abd El-Salam Khalil; Mohammed Ali Akeila

2025, Vol: 87

87Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

« Previous Article

Original Article

AJVS. 2013; 39(1): 112-121

Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle

Hazem Farouk Mahmoud, Gaber S. Abdellrazeq, Mahmoud M. El-naggar, Essam Amin Nasr, Samy Abd El-salam Khalil, Mohammed Ali Akeila.

Abstract
Bovine Tuberculosis an important infectious disease of cattle in developing countries which needs an urgent requirement for effective strategies for disease eradication. The spread of Bovine Tuberculosis also poses a human health risk. We assessed the possibility of using the quantification of IFN-ɣ-producing CD4+ lymphocytes by Cytokine Flow Cytometric analysis of intracellular IFN-ɣ expression for discrimination of naturally infected cattle "which is confirmed by isolation of M.bovis" from vaccinated animals with BCG vaccine. The obtained results showed that significant numbers of IFN-γ-expressing CD4+ T-cells were detected following culture of heparinized blood from M. bovis-infected animals with purified protein derived from M.bovis (PPD-B), the BCG-vaccinated animals had a significant low number of CD4+ T lymphocytes expressing IFN-ɣ compared with naturally M.bovis-infected animals. It is concluded that this assay could allow an accurate diagnosis of M. bovis and allow the discrimination of BCG-vaccinated cattle from infected cattle, which can evaluate the recent prepared vaccine used against tuberculosis in cattle. So it is recommended to evaluate this discrimination technique on larger number of animals to deduce dependable results

Key words: Bovine Tuberculosis, BCG vaccine, T-lymphocytes, Gamma Interferon

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Hazem Farouk Mahmoud Articles by Gaber S. Abdellrazeq Articles by Mahmoud M. El-Naggar Articles by Essam Amin Nasr Articles by Samy Abd El-Salam Khalil Articles by Mohammed Ali Akeila
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Mahmoud HF, Abdellrazeq GS, El-naggar MM, Nasr EA, Khalil SAE, Akeila MA. Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle. AJVS. 2013; 39(1): 112-121.

Web Style

Mahmoud HF, Abdellrazeq GS, El-naggar MM, Nasr EA, Khalil SAE, Akeila MA. Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle. https://www.alexjvs.com/?mno=43730 [Access: May 03, 2025].

AMA (American Medical Association) Style

Mahmoud HF, Abdellrazeq GS, El-naggar MM, Nasr EA, Khalil SAE, Akeila MA. Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle. AJVS. 2013; 39(1): 112-121.

Vancouver/ICMJE Style

Harvard Style

Mahmoud, H. F., Abdellrazeq, . G. S., El-naggar, . M. M., Nasr, . E. A., Khalil, . S. A. E. & Akeila, . M. A. (2013) Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle. AJVS, 39 (1), 112-121.

Turabian Style

Mahmoud, Hazem Farouk, Gaber S. Abdellrazeq, Mahmoud M. El-naggar, Essam Amin Nasr, Samy Abd El-salam Khalil, and Mohammed Ali Akeila. 2013. Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle. Alexandria Journal of Veterinary Sciences, 39 (1), 112-121.

Chicago Style

Mahmoud, Hazem Farouk, Gaber S. Abdellrazeq, Mahmoud M. El-naggar, Essam Amin Nasr, Samy Abd El-salam Khalil, and Mohammed Ali Akeila. "Efficacy of Cytokine Flow Cytometric Assay to Differentiate Between Tuberculosis Infected and BCG Immunized Cattle." Alexandria Journal of Veterinary Sciences 39 (2013), 112-121.

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article