E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources

May K. Zaghloul, Helmy A. Torky, Rania I. EL- meslemany.

Abstract
Klebsiella pneumoniae is zoonotic, Gram-negative bacteria belong to the family
Enterobacteriaceae. The primary goal of this study was to identify and characterize genetic relationship among K. pneumoniae that isolated from clinically infected broilers, human and mastitis milk, and expression of virulence factors, and antimicrobial resistance in multi-drug resistant (MDRs). A total of 88 klebsiella pneumoniae strains were isolated from different hosts (22 from broiler chickens, 48 human and 18 cows) during 2019 -2020 in El -Behira governorate. These strains were primary identified by microscopic examination, traditional biochemical tests and confirmed by using VITEK. Investigation of antibiotic susceptibility of identified isolates revealed that more than 70% of k .pneumoniae strains are multi-drug resistant. ESBL (Extended-spectrum beta-lactamase-producing k.pneumoniae) screening by using the double-disk synergy method and VITEK were determined (4388) (10chicken, 27human, and 6 cows). Capsule, hypermucoviscosity, and hemolytic activity were also, assayed phenotypically.
Results showed that Capsule, hypermucoviscosity, and hemolytic activity were detected in 100%, 30.7%, and 25%, respectively. Biofilm binding activity showed highly, moderately, and weakly adherent observed in 18.1%, 38.6%, and 18.2, respectively. The detection of two virulence genes: one encoding invasion (uge: uridine diphosphate galactose 4 epimerase) and the other encoding siderophores (kfu: iron uptake system) by using PCR technique reveal the existence of The uge gene in all examined isolates (n=15/15; 100%), while the kfu gene was detected in (n=2/5) broiler chicken and (n=3/5) human but absent at all from cow strains. Fifteen (15) MDR strains (5broilers, 5 human, and 5cows) that had high biofilm formation, ESBL, hypermucoviscosity, and capsule were investigated for their genetic relatedness using ERIC –PCR , showed that there is a more or less a narrow diversity among strains that are isolated from each same host (chicken or human or cows). However, the results indicated the occurrence of transverse infections between the three hosts. This fact to be confirmed it needs more investigation of many numbers of strains.

Key words: Klebsiella pneumoniae, virulence factors, biofilm formation, ERIC – PCR.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by May K. Zaghloul
Articles by Helmy A. Torky
Articles by Rania I. EL- meslemany
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Zaghloul MK, Torky HA, meslemany RIE. Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. AJVS. 2021; 71(1): 11-21. doi:10.5455/ajvs.77067


Web Style

Zaghloul MK, Torky HA, meslemany RIE. Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. https://www.alexjvs.com/?mno=77067 [Access: December 06, 2023]. doi:10.5455/ajvs.77067


AMA (American Medical Association) Style

Zaghloul MK, Torky HA, meslemany RIE. Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. AJVS. 2021; 71(1): 11-21. doi:10.5455/ajvs.77067



Vancouver/ICMJE Style

Zaghloul MK, Torky HA, meslemany RIE. Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. AJVS. (2021), [cited December 06, 2023]; 71(1): 11-21. doi:10.5455/ajvs.77067



Harvard Style

Zaghloul, M. K., Torky, . H. A. & meslemany, . R. I. E. (2021) Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. AJVS, 71 (1), 11-21. doi:10.5455/ajvs.77067



Turabian Style

Zaghloul, May K., Helmy A. Torky, and Rania I. EL- meslemany. 2021. Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 71 (1), 11-21. doi:10.5455/ajvs.77067



Chicago Style

Zaghloul, May K., Helmy A. Torky, and Rania I. EL- meslemany. "Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 71 (2021), 11-21. doi:10.5455/ajvs.77067



MLA (The Modern Language Association) Style

Zaghloul, May K., Helmy A. Torky, and Rania I. EL- meslemany. "Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 71.1 (2021), 11-21. Print. doi:10.5455/ajvs.77067



APA (American Psychological Association) Style

Zaghloul, M. K., Torky, . H. A. & meslemany, . R. I. E. (2021) Virulence Factors and Biofilm Formation of Klebsiella Pneumoniae Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 71 (1), 11-21. doi:10.5455/ajvs.77067





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles