E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi.

Abstract
A sero-prevalence survey of Toxoplasmosis in domestic animals (sheep, goats and camels) was conducted in the Najran region of Saudi Arabia from August to November 2014 to determine the status of the disease, as well as to evaluate the diagnostic performance of the serological tests used. A total of 263 sera were tested, 85 sheep; 88 goats and 90 camels, for the presence of Toxoplasma gondii antibodies using Indirect Hemagglutination test (IHAT) and enzyme-linked immunosorbent assay (ELISA). IHAT showed the higher prevalence of Toxoplasmosis (43.5% in sheep flowed by 31.8% in goats and 24.4% in camels) as compared with ELISA. On the other hand, ELISA showed the higher prevalence of Toxoplasmosis in sheep (45.9%) as compared with IHAT(43.5%). When the data from the IHAT was compared with that of the ELISA test, which was used as a reference test for toxoplasmosis, IHAT had the highest sensitivity (88.1%). It could be concluded that the IHAT and ELISA tests are efficient diagnostic tools for detection and selective diagnosis of Toxoplasmosis. Also high seroprevalence of Toxoplasma gondii in studied animals indicated the importance of these animals as the main source of human infection.

Key words: Najran, Saudi Arabia, Seroprevalence, Indirect Hemagglutination test, enzyme-linked immunosorbent assay, Toxoplasmosis.


 
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How to Cite this Article
Pubmed Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. AJVS. 2015; 46(1): 15-19. doi:10.5455/ajvs.184502


Web Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. http://www.alexjvs.com/?mno=184502 [Access: February 09, 2020]. doi:10.5455/ajvs.184502


AMA (American Medical Association) Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. AJVS. 2015; 46(1): 15-19. doi:10.5455/ajvs.184502



Vancouver/ICMJE Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. AJVS. (2015), [cited February 09, 2020]; 46(1): 15-19. doi:10.5455/ajvs.184502



Harvard Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi (2015) Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. AJVS, 46 (1), 15-19. doi:10.5455/ajvs.184502



Turabian Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. 2015. Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. Alexandria Journal of Veterinary Sciences, 46 (1), 15-19. doi:10.5455/ajvs.184502



Chicago Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. "Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay." Alexandria Journal of Veterinary Sciences 46 (2015), 15-19. doi:10.5455/ajvs.184502



MLA (The Modern Language Association) Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi. "Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay." Alexandria Journal of Veterinary Sciences 46.1 (2015), 15-19. Print. doi:10.5455/ajvs.184502



APA (American Psychological Association) Style

Mosa M. Bahnass, Ismail S. El-Shahawy, Elshaima M. Fawzi (2015) Comparative Analysis of Toxoplasmosis in Farm Animals by Indirect Hemagglutination Assay and Enzyme linked Immunosorbent Assay. Alexandria Journal of Veterinary Sciences, 46 (1), 15-19. doi:10.5455/ajvs.184502





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