E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman.

Abstract
40 milk samples were collected from cattle suffering from chronic mastitis with no or poor response to antibiotic therapy with common antibiotics, these samples were subjected tobacteriological and mycological culture then confirmed by traditional fungal confirmatory procedures and multiplex PCR (m-PCR). The resultsshowed 37 positive samples for fungal culture hence C. albicans took the lead of them by 60 % , A. fumigatus 22.5% , A. niger 10 % while C. neoformans not detected in any sample. Mixed bacterial and fungal infectionwas detected in 83.78% whilefungi as a sole etiological agent were detected in 16.22% of samples. m PCR succeeded to distinguish between A. fumigatus , A. niger and C. albicans more accurate and fast than traditional confirmatory methods .Pure mycotic mastitis cases were subjected to treatment by a new novel drug consisting of extract of medicinal plants (garlic and sesame oil). All treated cases responded well to this drug and returned to normal udder condition and to full milk production.

Key words: PCR, candida,Asperigillus, Mycotic Mastitis.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Esraa M. Bakr
Articles by Abd El-kareem M. Abd El-Tawab
Articles by Tharwat M. Elshemey
Articles by Amir H. Abd-Elrhman
on Google
on Google Scholar
Article Statistics
 Viewed: 4891
Downloaded: 847


REFERENCES
Abd El-Razik, Kh. A., Khaled, A., Abdelrahman , Sherein, Abd El-Moez, Enas, N. Danial. 2011. New approach in diagnosis and treatment of Bovine Mycotic Mastitis in Egypt. Afr. J. Microbiol. Res. 5 (31): 5725-5732. [DOI via Crossref]   
Akodouch, L., Aissi, M., Zenia, S., Saadi, A. 2014. Importance of Yeasts in the Mammary Infection of the Cattle in the Region of Sidi M’Hamed Ben Ali, Wilaya of Relizane, Algeria. J. Vet. Sci. Technol. 5: 2-7.
Al-Ameed, A. I. 2013. Isolation and identification of fungi from infected milk samples obtained from cattle with mastitis and studying the antifungal activity of Rosemary Ethanolic extract agaimst the main strains. Diyala Agricultural Sci. J. 5 (2) 1-13.
Amsaveni, S.S., Ramesh Hariharan, 2012. Anticandidal activity of plant extracts against bovine mastitis isolates .Tamilnadu J. veterinary and animal sciences 8(2): 72-75.
Awad, F.I., El-Molla, A., Fayed, A., Abd-El-Halim, M., Refai, M. 1980. Studies on mycotic mastitis in Egypt. Vol. 40 No. 3 pp. 35-41.
Bourtzi-Hatzopoulou, E., Zdragas, A., Petridou, E., Filiousis, G. 2003. Yeasts as a causative agent of bovine mastitis in Greece. J. Hellenic Vet. Med. Society 54 (2): 105-110.
Brian C. Foster. T. 2001. An In Vitro Evaluation of Human Cytochrome P450 3A4 and P-glycoprotein Inhibition by Garlic. J. Pharm. Pharmaceut. Sci. 4(2):176-184.
Chong-Kuei Lii, Kai-Li Liu, Yi-Ping Cheng, Ai-Hsuan Lin, Haw-Wen Chen, Chia-Wen Tsai. 2010. Sulforaphane and α-Lipoic Acid Upregulate the Expression of the π Class of Glutathione S-Transferase through c-Jun and Nrf2 Activation . J. Nutrit. l. 140 (5): 885-892. [DOI via Crossref]    [Pubmed]   
Davis, S.R., 2005. An overview of the antifungal properties of allicin and its breakdown products--the possibility of a safe and effective antifungal prophylactic. Mycoses. 48 (2):95-100. [DOI via Crossref]    [Pubmed]   
Santos R., Marin J.M. 2005. Isolation of Candida spp. from mastitic bovine milk in Brazil. Mycopathol. 159 (2): 251-253. [DOI via Crossref]    [Pubmed]   
Deb, R., Kumar, A., Chakraborty, S., Verma, A.K., Tiwari, R., Dhama, K., Singh, U., Kumar, S. 2013. Trends in diagnosis and control of bovine mastitis: a review. Pak. J. Biol. Sci. 16 (1) 1653-1661. [DOI via Crossref]    [Pubmed]   
Esra S., Erhan, O. 2011. In vitro biofilm activity of candida species isolated from Anatolian buffaloes with mastitis in western turkey. Vet. Arhiv. 81 (6):723 -730.
Geoffrey, P., Fanrong, K., Ying, S., Hui, W., Catriona H., Tania, C. S. 2006. Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization J. Clin. Microbiol. 44 (3): 876-880. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Geraldo, M. C., Ulisses, de P., Maria, Aparecida, G., Nivaldo, da S., 2012 Yeast mastitis outbreak in a Brazilian dairy herd Braz. J. Vet. Res. Anim. Sci., São Paulo, 49 (3): 239-243.
Ghodasara, S. N., Savsani, H. H., Vataliya, P.H. 2012. Use of pvp-iodine for therapeutic management of clinical case of mastitis in jaffrabadi buffaloes and gir cows. Buffalo bulletin.31 (3)
Gonzalez, R.N. 1996. Prototheca, Yeast, and Bacillus as a Cause of Mastitis National Mastitis Council Annual Meeting, Page 82.
Henk, H., Satu, P., Karin, P., Waller, J. S., Hogan, Theo J .G.M. Lam, Stephen P. Oliver, Ynte H Schhukken, Herman W. Barkema, J. Eric Hillerton. 2011. Current status and future challenges in mastitis research NMC Annual meeting proceeding.
Katey, M. Lemar, Ourania Passa, Miguel A. Aon, Sonia Cortassa, Carsten T. Müller, Sue Plummer, Brian O’Rourke, David Lloyd 2009. Allyl alcohol and garlic (Allium sativum) extract produce oxidative stress in Candida albicans. Microbiol. 151: 3257-3265.
Keane, O.M., Budd, K.E., Flynn, J., McCoy, F. 2013. Increased detection of mastitis pathogens by real-time PCR compared to bacterial culture. Vet. Rec. 173 (11):268. [DOI via Crossref]    [Pubmed]   
Kovacs, N. 1928. A simplified method for detection In dole formation by bacteria . Z. Immunostats for such, Chem.Abst., 22:3425.
Krukowski, H., Lisowski, A., Rózański, P., Skórka, A. 2006. Yeasts and algae isolated from cows with mastitis in the south-eastern part of Poland. Pol. J. Vet Sci. 9 (3):181-184. [Pubmed]   
Londhe, V. P., Gavasane, A.T., Nipate, S.S., Bandawane, D.D., Chaudhari, P.D. 2011. Role of Garlic (ALLIUM SATIVUM) in various diseases:an overview . J. Pharmaceut. Res. Opinion 1 (4) 129 - 134.
Ludmilla, S., Silvio, L., Marília, J. F., Margarete, R., Marialice, P. C. 2011. Aerobic and anaerobic bacteria and Candida species in crude milk J. Microbiol. Antimicrob. 3 (8): 206-212.
Luo, G., Mitchell, T.H. 2002. Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR journal of clinical microbiology p. 2860-2865. [Pubmed]    [PMC Free Fulltext]   
Mansourian, A., Boojarpour, N., Ashnagar, S., Momen Beitollahi, J., Shamshiri, A.R. 2014. The comparative study of antifungal activity of Syzygium aromaticum, Punica granatum and nystatin on Candida albicans; an in vitro study. J. Mycol. Med. 24 (4):e163-168. [DOI via Crossref]    [Pubmed]   
Masoud, T. G., Ali, R. K., Saeideh, P. 2012. Milk mycoflora survey of dairy cows with or without mastitis Iranian J. Vet. Sci. Technol. 4 (1): 69-74.
Mayr, A., Lass-Flörl, C. 2011. Epidemiology and antifungal resistance in invasive aspergillosis according to primary disease - review of the literature Eur. J. Med. Res. 16:153-157. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Nopamart Trakranrungsie. 2011. Plant derived antifungals-trends and potential applications in veterinary medicine :Amini review. A. Mendez-vilas (Ed.).
Pachauri, S., Puneet, V., Sandeep, K. D., Manoj, K. G. 2013. Involvement of fungal species in bovine mastitis in and around Mathura, India vet. world. 393-395.
Patwardhan, B., Mashelkar, R.A. 2009. Traditional medicine-inspired approaches to drug discovery: can Ayurveda show the way forward? Drug Discov Today. (15-16):804-411. [DOI via Crossref]    [Pubmed]   
Pengova. 2002. A prevalence of mycotic mastitis in cows. Acta Vet. (Beograd), 52 (2-3):133-136.
Quinn, P.J., Markey, B.K. Carter, M.E., Donnelly, W.J.C., Leonard, C.F. Vet Microbiology and Microbial Disease. Textbook, MPG Books LTd, Bodmin, Cornwall P. 111. 2002.
Radostitis, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D. Veterinary Medicine, 10 th Ed. Saunders Elsevier philadelphia, USA.2007.
Rayaz, A., Darand, B.G. 2013. Present Status, Future Road Map and Diagnosis of Zoonotic Mycotic Diseases Biomed. Pharmacol. J. 6 (2): 467-470.
Sambrook J, Fritsch EF, Maniatis T. Moleculer cloning: A laboratory manual, 2nd ed. Cold Spring Harbor lab., N.Y. 1989.
Scaccabarozzi, L., Locatelli, C., Pisoni, G., Manarolla, G., Casula, A., Bronzo, V., Moroni, P. 2011. Short communication: Epidemiology and genotyping of Candida rugosa strains responsible for persistent intramammary infections in dairy cows. J Dairy Sci. 94 (9):4574-4577. [DOI via Crossref]    [Pubmed]   
Suhyla, T., Seyhan, K. 2010. The slime production by yeasts isolated from subclinical mastitic cows. Acta Vet. Brno, 79: 581-586. [DOI via Crossref]   
Sukumar, K., James, P.C. 2012. Incidence of fungal mastitis in cattle. Tamilnadu J. Vet. Anim. Sci. 8 (6) 356 - 359.
Władysław, W., Mariola, B., Tomasz, P. 2010. Yeast mastitis in dairy cows in the middle-eastern part of Poland Bull Vet. Inst. Pulawy 54: 201-204.
Zaragoza, S., Carolina Cervantes Olivares, Roberto Arnulfo, Ducoing Watty, Andrés Ernesto;, de la Pe-a Moctezuma, Alejandro; Villa Tanaca, Lourdes. 2011. Yeasts isolation from bovine mammary glands under different mastitis status in the Mexican High Plateu. Revista Iberoamericana de Micología Rev. Iberoam. Micol. 28:79-82. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. AJVS. 2015; 46(1): 138-145. doi:10.5455/ajvs.189682


Web Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. http://www.alexjvs.com/?mno=189682 [Access: February 09, 2020]. doi:10.5455/ajvs.189682


AMA (American Medical Association) Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. AJVS. 2015; 46(1): 138-145. doi:10.5455/ajvs.189682



Vancouver/ICMJE Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. AJVS. (2015), [cited February 09, 2020]; 46(1): 138-145. doi:10.5455/ajvs.189682



Harvard Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman (2015) Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. AJVS, 46 (1), 138-145. doi:10.5455/ajvs.189682



Turabian Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. 2015. Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. Alexandria Journal of Veterinary Sciences, 46 (1), 138-145. doi:10.5455/ajvs.189682



Chicago Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. "Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle." Alexandria Journal of Veterinary Sciences 46 (2015), 138-145. doi:10.5455/ajvs.189682



MLA (The Modern Language Association) Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman. "Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle." Alexandria Journal of Veterinary Sciences 46.1 (2015), 138-145. Print. doi:10.5455/ajvs.189682



APA (American Psychological Association) Style

Esraa M. Bakr, Abd El-kareem M. Abd El-Tawab, Tharwat M. Elshemey, Amir H. Abd-Elrhman (2015) Diagnostic and Therapeutic Studies on Mycotic Mastitis in Cattle. Alexandria Journal of Veterinary Sciences, 46 (1), 138-145. doi:10.5455/ajvs.189682





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 8 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 7 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 5 times [Click to see citing articles]

  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]