E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem.

Abstract
This work was attempted to study the occurrence of BHV-1 as a serious viral pathogen in the genitalia of she camels. Out of 120 female genitalia (vulva-vagina-cervix and uterus), collected from different slaughter houses, 13 (10.83%) samples contained suspected viral agents which induced cytophathic effects on MDBK cell line. Five out of 13 suspected viral isolates could be completely neutralized using virus neutralization test (VNT). Seven out of 13 CPE positive viral agents were confirmed to be BHV-1 by PCR assay. IFAT revealed the presence of BHV-1in the epithelial lining of the vulva and vagina of BHV-1 positive tissue specimen. The percentage of positive serum reactors was 17 (14.16%). Gross examination of BHV-1 positive samples showed swollen endometrium, congested vulva with apparent laceration and papules. The vagina showed congested mucosa with multiple vesicles. Histopathlogical examination of the genitalia of the affected she camels revealed vulvitis, vaginitis, cervicitis and endometritis. In conclusion, the occurrence of the genital form of BHV-1 (infectious pustular vulvovaginitis, IPV) in she camels was declared. Accordingly, the role played by BHV-1 in causation of infertility and abortion in she camels should be investigated. Also, attention must be paid toward the possibility of infected she camels to transmit such virus infection to farm animals in contact.

Key words: She camels, BHV-1, genitalia, CPE, PCR, IFAT, Pathological findings.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Samah F. Darwish
Articles by Thanaa K. Hassanien
Articles by Halaa A. Salem
on Google
on Google Scholar
Article Statistics
 Viewed: 4134
Downloaded: 606


REFERENCES
Abraham, A., Ayakon, N., Marcus, S. 1982. An outbreak of IBR/IPV in bulls and dairy cattle in Israel. Clinical and diagnostic aspects. Refuel Vet. 39: 95-98.
Åkerstedt, J., Nordstrom, M., Market, T. 2012. The surveillance programme for infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis (IPV) in Norway. Norwegian Veterinary Institute Ullevålsveien 68, BP, 750.
Anne-Brit, H., kaminjono, j. 2013. Infectious Bovine Rhinotracheitis-Infectious Bovine vulvovaginitis viral isolated from cattle with vaginitis and epididymitis. Am. J. Vet. Res. 36:123-125.
Bancroft, J. D., Stevens, A. 1990. Theory and Practice of histological Techniques. 3rd edition, New York.
Brian, W.J.M., Hiller, O.K. 1996. Biosafety in virology laboratory. Virology Methods Manual pp: 357-362.
Constable, N. 2007. Seroprevalence of infectious bovine rhinotracheitis in Gujarat State. M. V. Sc. Thesis submitted to Gujarat Agriculture University, Sardarkru shinagar.
Costa, E.O. 2002. Influence of infections and infectious disease on buffaloes reproduction. Proc. First Buffalo Symposium of Americas, Brazil, Belem, Para: 5-14. [Pubmed]   
Darwish, S. F., Amin, A.S. 2011. Evaluation of Conventional PCR assay in comparison to Real-time PCR Assay for Detection of Bovine Herpesvirus-1 in Bovine Semen and Seminal Plasma. Assiut Vet . J. 57: 285-299.
Dunn, S., Godwin, J., Hoare, R. J., Kirkland, P. 1994. Diseases of feedlot cattle: project DAN 64.Final report to the Meat Research Corporation, Sydney, N. S. W. Edwards, S., White, H., Nixon, P. 1990. The virulence of British isolates of BHV-1 in relationship to viral genotype. Brit. Vet. J. 147: 216-231.
Ghoniem, H.A., Saad, A. M., Hala, A. S., Ibrahim, A. A. 2011. Staphylococcus aurous protein-A as a rapid and sensitive test in comparative with other serological tests for detection of Rift valley fever antibodies in vaccinated camels. B.S. Vet. Med. J. 21:67-72.
Gilbert, R. O., Oettle, E. E. 1990. An outbreak of granulomatous vulvitis in feedlot heifers. South Afr. Vet. Assoc. 61: 41-43.
Hage, A.M., Whit beck, J.C., Knapp, A.C., Unquiet, L.W., Lawrence, W.C., Bello, L.J. 1996. Synthesis, processing, and oligomerization of bovine herpes virus 1 gE and gI membrane proteins. J. Virol. 70: 78-84.
Ibrahim, S.P., Fatima, I., Sahara, A.A 1983. Isolation of IBR virus from buffaloes in Malaysia. Vet. Rec. 112: 303-304. [DOI via Crossref]    [Pubmed]   
Intisar, K.S., Ali, Y.H., Khalafalla, A.I., Rahman Mahasina, E.A. and Amin, A.S. 2009. Natural exposure of Dromedary camels in Sudan to infectious bovine rhinotracheitis virus (bovine herpes virus-1). Acta Tropica 111: 243-246. [DOI via Crossref]    [Pubmed]   
Isa, M. I. 1998. Serological survey against some viral diseases in camels in Sharkia Governorate, Egypt. In: Proceedings of the Third Annual Meeting for Animal Production Under Arid Conditions, United Arab Emirates University, pp. 167-173.
Jain, L. A.N., Kanani, T.J. P, Purohit, J. H., Jhala, M.K., Chuahan H.C., Chand, B.S. 2008. Seroprevalence of bovine herpes virus 1 (BHV-1) in Indian breeding bulls of Gujarat. Buffalo Bull. 27:165- 169.
Kaashoek, M. J., Rijsewijk, F.A.M. and van Oirschot, J.T. 1996. Persistence of antibodies against BHV-1 and virus reactivation two to three years after infection. Vet. Microbiol. 53: 103-110. [DOI via Crossref]   
Karge, E. 1989. Infectious bovine rhinotracheitis / Infectious pustular vulvovaginitis (IPR/IPV) In: Applied Veterinary Epidemiology, Elsevier Science Publishers, Amsterdam, New York, 10: 145 - 148.
Khans, R.F., Gibbs, E.P.J., Larsen, R.E. 1980. The search for viruses in bovine semen, a review. Theriogenol. 14: 151-165. [DOI via Crossref]   
Lassie, N., Erika, A., Mauro, H. 2007. Epidemiology and eradication of infectious bovine rhinotracheitis /infectious pustules vulvovaginitis virus (IBR/IPV) in Finland. Act. Vet. Scand. 49: 3. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Mohamed, K. E., Ahmad, M.A., Ahmed, M.A., Effat, L.E. 2013. Investigation of the Immune Status of Camels (Camelus dromedaries) Against Some Viral Diseases. Alex. J. Vet. Sci. 39:12-17.
Monge, A., Elvira, L., Gonzalez, J.V., Azotizes, M., Wallenberg, G.J. 2006 Bovine herpes virus 1-associated postpartum metritis in a Spanish dairy herd. Res. Vet. Sci. 80:120-25. [DOI via Crossref]    [Pubmed]   
Moussa, A.A., M.S., Saber, E. N., Shalaby, W.N., Ayoub, S. E., Mohsen, M.M., Madbouly, A.A., El Sanousi, M.M., Fathia, A. S., Reda, I.M. 1990. Serological survey on the prevalence of BHV1 in Domestic Animal in Egypt. Vet. Med. J. Giza, 38: 87-94.
Muylkens, B., Thiry, J., Kirten, P., Schynts, F., Thiry, E. 2007. Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Vet. Res., 38: 181-209. [DOI via Crossref]    [Pubmed]   
OIE 2004. Manual of diagnostic tests and vaccines for terrestrial animals. 5th edition.
OIE 2010. Manual of diagnostic tests and vaccines for terrestrial animals. 5th edition.
Rachel, N. 2010. Infectious Bovine Rhinotracheitis / Infectious Pustular Vulvovaginitis. National Animal Health Information System, Animal Health Australia (AHA), developed by Aus. Vet. Anim Health Serv., 2: 45.
Radostits, O. M., Gay, C. C., Blood, D. C. and Hinchcliff, K. W. 2000. Infectious bovine rhinotrcheitis: In veterinary Medicine A textbook of the diseases of cattle, sheep, pigs, goats and horses".9 th Edit. B. Saunders Company Ltd. London, New York, Philadelphia, San Francisco St Louis, Sydney.
Radostits, O. M., Gay, C. C., Hinchcliff, K. W.,Constable, P.D. 2007. Infectious bovine rhinotracheitis (IBR, Red Nose), bovine Herpesvirus-1(BHV-1) infection. In veterinary Medicine. 10th ed. Saunders, El-Sevier. 1349-1361.
Rimstad, S., Consalvo, F., D'Onofrio, G., Capucci, L., Cabassi, C.S., Allegri, G., Bigliardi, E.,Civardi, A., Flammini, C.F. 1992. A serological survey of different bovine herpes viruses (BHV-1, BHV-2, BHV-4) in dairy buffaloes of southern and northern Italy. Proc. Fifth World Buffalo Congress, October 13-16, Caserta, Italy: 626-630.
Showdown, W.A. 1994. Infectious Bovine rhinotracheitis and infectious pustular vulvovaginitis in Australian cattle. Aust. Vet. J. 40: 277-288.
Straub, O.C. 2001. Advances in BHV-1 (IBR) research. Dtsch Tierarztl Wochenschr 108: 419-422. [Pubmed]   
Toussaint, J.F., Dubois, A., Dispas, M., Paquet. D., Letellier, C., Kerkhofs, P. 2007. Infectious bovine rhinotracheitis virus, Infectious pustular vulvovaginitis. FAO inter-regional cooperative research network in buffalo food and agriculture organization of the United Nations regional office for Europe.
Van Oirschot, F.A.M, Van Engelenburg, F.A.C., Kaashoek, M.J., Van den Berg L., Moerman, A. and Van Oirschot, J.T. 1993. A glycoprotein E deletion mutant of bovine herpes virus 1 is a virulent in cattle. J. Gen. Viral., 75: 2311-2318.
Wang, J., O’Keefe, J., Della Orr, D., Loth, L., Banks, M., Wakeley, P., West, D., Card, R., Ibata, G., Van Maanenc, K., Thoren, P., Isaksson, M., Pierre Kerkhofs, P. 2007. Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen. J. Virol. Methods 144, 103-108. [DOI via Crossref]    [Pubmed]   
Wood, D. J., Corbett, G. 1982. Use of FITC-protein- A in place of fluorescent-conjugated anti-gamma globulins for rapid virus diagnosis by immunofluorescence. J. Clin. Patho. 35(4): 472-475. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Yousseif, N.M., Gabry, G.H., Hussein, M., Omayma, A.A.S. 2003. Occurrence of Parainfluenza type 3 and bovine herpes virus type 1 (BHV-1) viruses (mixed infection) among camels. Egypt. J. Agric. Res. 81: 781-791.

How to Cite this Article
Pubmed Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. AJVS. 2015; 46(1): 130-137. doi:10.5455/ajvs.192093


Web Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. http://www.alexjvs.com/?mno=192093 [Access: February 08, 2020]. doi:10.5455/ajvs.192093


AMA (American Medical Association) Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. AJVS. 2015; 46(1): 130-137. doi:10.5455/ajvs.192093



Vancouver/ICMJE Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. AJVS. (2015), [cited February 08, 2020]; 46(1): 130-137. doi:10.5455/ajvs.192093



Harvard Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem (2015) Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. AJVS, 46 (1), 130-137. doi:10.5455/ajvs.192093



Turabian Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. 2015. Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. Alexandria Journal of Veterinary Sciences, 46 (1), 130-137. doi:10.5455/ajvs.192093



Chicago Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. "Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels." Alexandria Journal of Veterinary Sciences 46 (2015), 130-137. doi:10.5455/ajvs.192093



MLA (The Modern Language Association) Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem. "Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels." Alexandria Journal of Veterinary Sciences 46.1 (2015), 130-137. Print. doi:10.5455/ajvs.192093



APA (American Psychological Association) Style

Samah F. Darwish, Thanaa K. Hassanien, Halaa A. Salem (2015) Patho-Molecular Studies on the Occurrence of Bovine Herpes Virus Type 1 in the Genitalia of She Camels. Alexandria Journal of Veterinary Sciences, 46 (1), 130-137. doi:10.5455/ajvs.192093





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 8 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 7 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 5 times [Click to see citing articles]

  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]