E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens

Ahmed M. Elshwehy, Samy A. Khalil, Helmy A. Turky, Rasha G. Tawfik.

Abstract
A total of 170 pus swabs samples were collected: (60 samples from multiple abscesses in sheep, 50 from lung abscess in young calf from abattoir and 60 from metritis cattle) for isolation and identification of Corynebacterium pseudotuberculosis. Out 18 isolates from 60 samples of multiple abscesses in sheep in a percentage (30%), out 6 isolates from 50 samples of lung abscess young calf in a percentage (12 %) and finally out 10 isolates from 60 samples of metritis from cattle in a percentage (16.7%). Thirty-four isolates of Corynebacterium pseudotuberculosis isolated from different samples were tested for their sensitivity to 10 antimicrobial agents. The isolates were highly sensitive to Vancomycin (VA) Streptomycin (S), moderately sensitive to Gentamicin (CN), Erythromycin (E), Tetracycline (TE), and Chloramphenicol (C) but were resistant to Cephotaxime (CTX), Penicillin (P), Rifampicin (RD) and Amoxycillin-Clavulenic acid (AMC). 12 isolates of Corynebacterium pseudotuberculosis has no ability to form biofilm (35.3%), 5 isolates has ability to form weak biofilm (14.7%), 13 isolates has ability to form moderate biofilm formation (38.2%), finally there are 4 isolates has ability to form strong biofilm formation (11.8%). Nine isolates of Corynebacterium pseudotuberculosis were tested by PCR for detection of sigma factor; only 2 isolates (one from lymph node abscess of sheep and another from lung tissue abscess of cattle) have virulent gene (sigma factor) with size 490 bp.

Key words: Corynebacteria pseudotuberculosis, biofilm, antibiotic sensitivity, sigma factor.


 
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How to Cite this Article
Pubmed Style

Elshwehy AM, Khalil SA, Turky HA, Tawfik RG. Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. AJVS. 2021; 68(1): 82-89. doi:10.5455/ajvs.205341


Web Style

Elshwehy AM, Khalil SA, Turky HA, Tawfik RG. Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. http://www.alexjvs.com/?mno=205341 [Access: February 26, 2021]. doi:10.5455/ajvs.205341


AMA (American Medical Association) Style

Elshwehy AM, Khalil SA, Turky HA, Tawfik RG. Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. AJVS. 2021; 68(1): 82-89. doi:10.5455/ajvs.205341



Vancouver/ICMJE Style

Elshwehy AM, Khalil SA, Turky HA, Tawfik RG. Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. AJVS. (2021), [cited February 26, 2021]; 68(1): 82-89. doi:10.5455/ajvs.205341



Harvard Style

Elshwehy, A. M., Khalil, . S. A., Turky, . H. A. & Tawfik, . R. G. (2021) Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. AJVS, 68 (1), 82-89. doi:10.5455/ajvs.205341



Turabian Style

Elshwehy, Ahmed M., Samy A. Khalil, Helmy A. Turky, and Rasha G. Tawfik. 2021. Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. Alexandria Journal of Veterinary Sciences, 68 (1), 82-89. doi:10.5455/ajvs.205341



Chicago Style

Elshwehy, Ahmed M., Samy A. Khalil, Helmy A. Turky, and Rasha G. Tawfik. "Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens." Alexandria Journal of Veterinary Sciences 68 (2021), 82-89. doi:10.5455/ajvs.205341



MLA (The Modern Language Association) Style

Elshwehy, Ahmed M., Samy A. Khalil, Helmy A. Turky, and Rasha G. Tawfik. "Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens." Alexandria Journal of Veterinary Sciences 68.1 (2021), 82-89. Print. doi:10.5455/ajvs.205341



APA (American Psychological Association) Style

Elshwehy, A. M., Khalil, . S. A., Turky, . H. A. & Tawfik, . R. G. (2021) Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens. Alexandria Journal of Veterinary Sciences, 68 (1), 82-89. doi:10.5455/ajvs.205341





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