E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt.

Amira Hussein El-Baz, Mona Mohieldin Elsayed.

Abstract
L. monocytogens is one of the major food-borne pathogens which can affect humans and a wide variety of animal species. It usually invades Milk and its products at several steps in the food chain, Listeriosis outbreaks remain an important problem globally. High resistance of L. monocytogens to the most of antibiotics due to misusing of it in both veterinary and human field, it necessitates searching about alternatives. Drug laden nanoparticles possibly will overwhelm the restrictions of conservative antibiotic actions related with poisonousness, unsuitable distribution, or enzymatic dilapidation. The aim of this research is to detect prevalence of this bacteria in farm milk and some milk products and to mark the result of H2O2 loaded on silver nanoparticles on Multidrug resistance strains of L. monocytogenes. Therefore, One hundred samples (25 farm bulk tank, 25 plain yoghurt and 50 fresh soft cheese (Talaga)) were haphazardly composed from dairy farms and different shops in different areas of Mansoura city, Dakahlia governorate. Egypt. The isolated strains of L. monocytogenes were evaluated phenotypically and genotypically for detection of virulence factors (invasive linked protein (iap), haemolysin (hylA) and actin polymerization protein (actA)) that were carried out by using multiplex PCR. Out of 100 samples 7 listeria monocytogenes isolates from Fresh soft cheese (Talaga) samples (14%). These 7 isolates had iap gene, while 6 isolates had hly gene and 5 isolates had actA gene. Also the three types of virulence genes were in four isolates at which a higher multiple antibiotic resistance between L.monocytogenes isolates. Assessment of antibacterial action of silver nanoparticles and hydrogen peroxide Antibacterial doings was assessed by broth micro-dilution test and our results revealed that H2O2/AgNPs with 6.25-3.13 g/mL MIC values of the original concentrations 13 g/mL MIC values of the original concentrations highly effective against L. monocytogens.

Key words: Egypt, Farm milk, H2O2 loaded on silver nanoparticles, Listeria monocytogens


 
ARTICLE TOOLS
Abstract
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Amira Hussein El-Baz
Articles by Mona Mohieldin Elsayed
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

El-Baz AH, Elsayed MM, . Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt..


Web Style

El-Baz AH, Elsayed MM, . Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt.. http://www.alexjvs.com/?mno=30487 [Access: January 17, 2021].


AMA (American Medical Association) Style

El-Baz AH, Elsayed MM, . Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt..



Vancouver/ICMJE Style

El-Baz AH, Elsayed MM, . Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt..



Harvard Style

El-Baz, A. H., Elsayed, M. M. & (0) Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt..



Turabian Style

El-Baz, Amira Hussein, Mona Mohieldin Elsayed, and . 0. Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt..



Chicago Style

El-Baz, Amira Hussein, Mona Mohieldin Elsayed, and . "Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt.."



MLA (The Modern Language Association) Style

El-Baz, Amira Hussein, Mona Mohieldin Elsayed, and . "Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt.."



APA (American Psychological Association) Style

El-Baz, A. H., Elsayed, M. M. & (0) Prevalence of Listeria monocytogens in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H2O2 loaded on silver nanoparticles in Dakahlia governorate. Egypt..





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]