E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail.

Abstract
Fibrin sealant, or fibrin "glue," is a unique surgical haemostatic, sealant and adhesive material that is being used in a variety of surgical situations. It is a two-component system in which a solution of concentrated fibrinogen and factor XIII are combined with a solution of thrombin and calcium to form a coagulum, simulating the final stage of the clotting cascade. The present study was designed to examine the adhesive effect of ethanol extracted fibrinogen of bovine origin on rat liver wound healing. In the present study fibrinogen was extracted from bovine plasma using cold ethanol and prothrombin was extracted from bovine plasma by exposure to barium chloride. The study was conducted on sixteen mature white male rats. They were allocated into two equal groups (8 rats /group) and a surgical liver wound was induced in the left liver loop of each rat. In the first group (control) the wounds were exposed to slight manual compression with a sterile cotton tampon and then left for natural healing and in the second group (fibrin glue treated) the wounds were sealed using a combination of (bovine fibrinogen, prothrombin and thromboplastin calcium). After 3 and 6 days of wound induction four rats of each group were euthanized under light ether anesthesia, their abdomens were excised and livers were collected, washed by cold saline, grossly examined and fixed in neutral formalin for histopathological examination. Also blood samples were collected at the days 3 and 6 of experiment to obtain serum for biochemical estimations of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate Aminotransferase (AST), total protein, albumin , globulin , cholesterol ,high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL).Fibrin glue treated liver wound ,exhibited a grossly marked uniform healing which was confirmed by histopathological examination indicated by the presence of organized fibrous tissue at the site of wound and the adjacent part of liver tissue, normally appeared without degeneration. While in control group the site of liver wound showed uniform healing and there histopathological examination revealed unorganized fibrous tissue formation and the adjacent part of liver tissue exhibits variable degrees of degeneration. The liver function parameters in control group were adversely affected significantly compared to fibrin glue treated group.

Key words: liver, fibrin glue, wound, Haemostasis, Hemorrhage.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Bahig R. Nematallah
Articles by Shereen B. Gad
Articles by Kariman A. Esmail
on Google
on Google Scholar
Article Statistics
 Viewed: 3097
Downloaded: 406


How to Cite this Article
Pubmed Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. AJVS. 2018; 56(2): 29-38. doi:10.5455/ajvs.291349


Web Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. http://www.alexjvs.com/?mno=291349 [Access: February 09, 2020]. doi:10.5455/ajvs.291349


AMA (American Medical Association) Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. AJVS. 2018; 56(2): 29-38. doi:10.5455/ajvs.291349



Vancouver/ICMJE Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. AJVS. (2018), [cited February 09, 2020]; 56(2): 29-38. doi:10.5455/ajvs.291349



Harvard Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail (2018) The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. AJVS, 56 (2), 29-38. doi:10.5455/ajvs.291349



Turabian Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. 2018. The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. Alexandria Journal of Veterinary Sciences, 56 (2), 29-38. doi:10.5455/ajvs.291349



Chicago Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. "The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats." Alexandria Journal of Veterinary Sciences 56 (2018), 29-38. doi:10.5455/ajvs.291349



MLA (The Modern Language Association) Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail. "The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats." Alexandria Journal of Veterinary Sciences 56.2 (2018), 29-38. Print. doi:10.5455/ajvs.291349



APA (American Psychological Association) Style

Bahig R. Nematallah, Shereen B. Gad, Kariman A. Esmail (2018) The Adhesive Properties of Fibrin Glue Extracted from Bovine Plasma on Liver Wound Healing in Male Rats. Alexandria Journal of Veterinary Sciences, 56 (2), 29-38. doi:10.5455/ajvs.291349





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 8 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 7 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 5 times [Click to see citing articles]

  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]