Abstract
Fibrin sealant, or fibrin "glue," is a unique surgical haemostatic, sealant and adhesive material that is being used in a variety of surgical situations. It is a two-component system in which a solution of concentrated fibrinogen and factor XIII are combined with a solution of thrombin and calcium to form a coagulum, simulating the final stage of the clotting cascade. The present study was designed to examine the adhesive effect of ethanol extracted fibrinogen of bovine origin on rat liver wound healing. In the present study fibrinogen was extracted from bovine plasma using cold ethanol and prothrombin was extracted from bovine plasma by exposure to barium chloride. The study was conducted on sixteen mature white male rats. They were allocated into two equal groups (8 rats /group) and a surgical liver wound was induced in the left liver loop of each rat. In the first group (control) the wounds were exposed to slight manual compression with a sterile cotton tampon and then left for natural healing and in the second group (fibrin glue treated) the wounds were sealed using a combination of (bovine fibrinogen, prothrombin and thromboplastin calcium). After 3 and 6 days of wound induction four rats of each group were euthanized under light ether anesthesia, their abdomens were excised and livers were collected, washed by cold saline, grossly examined and fixed in neutral formalin for histopathological examination. Also blood samples were collected at the days 3 and 6 of experiment to obtain serum for biochemical estimations of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate Aminotransferase (AST), total protein, albumin , globulin , cholesterol ,high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL).Fibrin glue treated liver wound ,exhibited a grossly marked uniform healing which was confirmed by histopathological examination indicated by the presence of organized fibrous tissue at the site of wound and the adjacent part of liver tissue, normally appeared without degeneration. While in control group the site of liver wound showed uniform healing and there histopathological examination revealed unorganized fibrous tissue formation and the adjacent part of liver tissue exhibits variable degrees of degeneration. The liver function parameters in control group were adversely affected significantly compared to fibrin glue treated group.

Key words: liver, fibrin glue, wound, Haemostasis, Hemorrhage.