E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model

Afrah Fatthi Salama, Mohammed A. Mansour, Wafaa M. Ibrahim, Eman S. Shalaan.

Abstract
Arsenic trioxide (As2O3) (ATO) and cisplatin (CIS) have potent antineoplastic effects in several types of cancer. Autophagy is important for normal cell function and survival, it is also used by tumor cells so, we studied it's role as possible mechanism of ATO and/or CIS antitumor effect on mice bearing Ehrlich ascites carcinoma (EAC) and checked whether ATO can enhance the antitumor potential of CIS. The study was carried out on eight groups of female mice; GP1 (negative control), GP2 (Erlich tumor only), GP3 (Normal +ATO), GP4 (Normal + CIS), GP5 (Normal + ATO+CIS), GP6 (EAC+ATO), GP7 (EAC+CIS) and GP8 (EAC +ATO+ CIS). In this study, viable cells were counted, percentage of viability (%) was calculated. Flowcytometry of autophagosome was appointed. Glutathione S-transferase (GST) and catalase (CAT) enzymes activities, total thiol and malondialdehyde (MDA) concentrations in liver tissues were determined to evaluate the effects of these drugs. Treatment with ATO (GP6) induced a significant decrease in tumor volume and percentage of viability with best result in combination of ATO with low dose of CIS (GP8) against EAC. Our results showed that a reduction in flourscence intensity of autophagosome marker that determined by flowcytometry especially in combination treated group (GP8). CAT, GST enzymes activities and total thiol level were decreased, while MDA level was increased in ATO treated group (GP6) and CIS treated group (GP7) as compared to EAC group. On other hand, combining both ATO and CIS in EAC treated group (GP8) decreased MDA level and augmented the level of total thiol and activities of CAT and GST enzymes. Therefore, our result revealed that combination of ATO and CIS have anti-tumor effects against EAC better than each one alone. So, we recommended the combination of ATO with CIS treatment due to their synergetic effect on cancer

Key words: Ehrlich ascites carcinoma – autophagy - arsenic trioxide - cisplatin.


 
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How to Cite this Article
Pubmed Style

Salama AF, Mansour MA, Ibrahim WM, Shalaan ES, . Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. AJVS. 2019; 61(1): 159-167. doi:10.5455/ajvs.29544


Web Style

Salama AF, Mansour MA, Ibrahim WM, Shalaan ES, . Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. https://www.alexjvs.com/?mno=29544 [Access: April 30, 2022]. doi:10.5455/ajvs.29544


AMA (American Medical Association) Style

Salama AF, Mansour MA, Ibrahim WM, Shalaan ES, . Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. AJVS. 2019; 61(1): 159-167. doi:10.5455/ajvs.29544



Vancouver/ICMJE Style

Salama AF, Mansour MA, Ibrahim WM, Shalaan ES, . Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. AJVS. (2019), [cited April 30, 2022]; 61(1): 159-167. doi:10.5455/ajvs.29544



Harvard Style

Salama, A. F., Mansour, M. A., Ibrahim, W. M., Shalaan, E. S. & (2019) Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. AJVS, 61 (1), 159-167. doi:10.5455/ajvs.29544



Turabian Style

Salama, Afrah Fatthi, Mohammed A. Mansour, Wafaa M. Ibrahim, Eman S. Shalaan, and . 2019. Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. Alexandria Journal of Veterinary Sciences, 61 (1), 159-167. doi:10.5455/ajvs.29544



Chicago Style

Salama, Afrah Fatthi, Mohammed A. Mansour, Wafaa M. Ibrahim, Eman S. Shalaan, and . "Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model." Alexandria Journal of Veterinary Sciences 61 (2019), 159-167. doi:10.5455/ajvs.29544



MLA (The Modern Language Association) Style

Salama, Afrah Fatthi, Mohammed A. Mansour, Wafaa M. Ibrahim, Eman S. Shalaan, and . "Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model." Alexandria Journal of Veterinary Sciences 61.1 (2019), 159-167. Print. doi:10.5455/ajvs.29544



APA (American Psychological Association) Style

Salama, A. F., Mansour, M. A., Ibrahim, W. M., Shalaan, E. S. & (2019) Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model. Alexandria Journal of Veterinary Sciences, 61 (1), 159-167. doi:10.5455/ajvs.29544





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