E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 
AJVS. 2013; 39(1): 145-154


Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates

Maged G. Hemida.

Abstract
Rotaviruses are large diverse group of viruses implicated in gastroenteritis in human, different animals and poultry species. They are among the common causes of infantile diarrhea worldwide. The emergence of rotaviruses is a very complicated process. Many rotaviruses can infect several species making their interspecies transmission quiet possible in many cases. The main goal of the current study was to detect the presence of group D avian rotavirus in clinical specimens from chicken farms in both Kafrelsheikh and Gharbia governorates. To achieve our goal we collected cloacal swabs, fecal specimens and pooled organs from several broiler chicken farms from both governorates. In the current study we reported the presence rotavirus in the cloacal swabs and tissue suspensions of pooled organs from chickens suffering from diarrhea. These results had been confirmed by different techniques such as the scanning electron microscope, the immunoelectron microscope, Dot ELISA and the hemagglutination test. Seroconversion of the affected chickens to that virus was documented by the hemagglutination inhibition test. For further confirmation we used a specific oligonucleotdes to amplify a conserved region in group D avian rotavirus VP-6 gene. Our results are showing high degree of identity of the detected viruses to the previously published sequences of group D-avian rotavirus VP-6 gene. This study confirmed for the first time the identification and molecular characterization of group D avian rotavirus in Delta region of Egypt.

Key words: Molecular, detection, group D, avian rotavirus


 
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REFERENCES
Bezerra, D.A., da Silva, R.R., Kaiano, J.H., Silvestre, R.V., de Souza Oliveira, D., Linhares, A.C., Gabbay, Y.B., Mascarenhas, J.D., 2012, Detection of avian group D rotavirus using the polymerase chain reaction for the VP6 gene. J Virol Methods. 185, 189-192.
Delana, Andreza, Melo, Bezerra, a, , René, Ribeiro, da, Silva, Melo Bezerra, D.A., da Silva, R. R, Kaiano, L et al, 2012, Detection of avian group D rotavirus using the polymerase chain reaction for the VP6 gene. J. Virol. Methods 85, 189-192.
Dusetty, P., Velazquez, F.R., Gutierrez-Escolano, A.L., Ludert, J.E., 2013, Evaluation of the second generation of a commercial latex agglutination test for the detection of rotavirus antigens in fecal samples. J. Clin. Virol. 57, 88-90.
Estes, M.K., Cohen, J., 1989, Rotavirus gene structure and function. Microbiol. Rev. 53, 410-449.
Ferreira, T.L., Becho, M. C, Bernardo, A, R, Chaves, Ribeiro, R. S, de Lima, J. S, Fialho, A. /m, Leit, J. G, 2006.Performance of a latex agglutination test in the diagnosis of acute gastroenteritis by rotavirus. Brazilian J Microbiology. 37, 587-589.
Gerna, G., Sarasini, A., Arista, S., di Matteo, A., Giovannelli, L., Parea, M., Halonen, P., 1990, Prevalence of human rotavirus serotypes in some European countries 1981-1988. Scand J Infect Dis. 22, 5-10.
Gough, R.E., Wood, G.W., Collins, M.S., Spackman, D., Kemp, J., Gibson, L.A., 1985, Rotavirus infection in pheasant poults. Vet Rec. 116, 295.
Johne, R., Otto,P.Roth,B.,Löhren,U.,Belnap,D.,Reetz,J.,Trojnar,E, 2011, Sequence analysis of the VP6-encoding genome segment of avian group F and G rotaviruses. Virology. 442, 384–439.
Kalica, A.R., Wyatt, R.G., Kapikian, A.Z., 1978, Detection of differences among human and animal rotaviruses, using analysis of viral RNA. J. Am. Vet. Med. Assoc. 173, 531-537.
Kapikian, A.Z., and Chanock, R. M, 1990, Rotaviruses. In :Fields BN, Knife DM, editors. Virology. 3d ed. New York: Raven Press, 1657-1708.
Lyb, V., Uliana, G, Valenzuela, C, Chalacon, J. L. V, Saidenberg, A. B. A, Sanches, A. A, Brandao, P. E, Jerez, J. A, Ferreria, A. J. P, 2006, Rotavirus detection and isolation from chickens with or without symptoms. Rev. Bras. Cienc. Avic. 8, 3. 187-191.
McNulty, M.S., Allan, G.M., Todd, D., McFerran, J.B., 1979, Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Arch Virol. 61, 13-21.
Minamoto, N., Oki, K., Tomita, M., Kinjo, T., Suzuki, Y., 1988, Isolation and characterization of rotavirus from feral pigeon in mammalian cell cultures. Epidemiol Infect. 100, 481-492.
Posada, D., 2008, Model Test: phylogenetic model averaging. Mol Biol Evol 25, 1253-1256.
Reynolds, D.L., Theil, K, Saif, Y. M, 1986, Demonstration of rotavirus and rota like virus in intestinal contents of diarrheic phesant chicks. Avian Dis. 31.
Rodger, S.M., Craven, J.A., Williams, I., 1975, Letter: Demonstration of reovirus-like particles in intestinal contents of piglets with diarrhoea. Aust. Vet. J. 51, 536.
Schumann, T., Hotzel, H., Otto, P., Johne, R., 2009, Evidence of interspecies transmission and reassortment among avian group A rotaviruses. Virology. 386, 334-343.
Takase, K., Nonaka, F., Sakaguchi, M., Yamada, S., 1986, Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures. Avian Pathol 15, 719-730.
Takehara, K., Kiuchi, H., Kuwahara, M., Yanagisawa, F., Mizukami, M., Matsuda, H., Yoshimura, M., 1991, Identification and characterization of a plaque forming avian rotavirus isolated from a wild bird in Japan. J Vet Med Sci. 53, 479-486.
Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007, MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 24, 1596-1599.
Thirumurugan, J., Kumanan, K, Venugopalanat, Nachimuthuk, 1997, Latex immunoassay for rapid detection of Newcastle disease virus Trop Anim Health Prod. 29, 227 - 230.
Woode, G.N., Bridger, J., Hall, G.A., Jones, J.M., Jackson, G., 1976, The isolation of reovirus-like agents (rota-viruses) from acute gastroenteritis of piglets. J Med Microbiol. 9, 203-209.
Wyatt, R.G., Greenberg, H.B., James, W.D., Pittman, A.L., Kalica, A.R., Flores, J., Chanock, R.M., Kapikian, A.Z., 1982, Definition of human rotavirus serotypes by plaque reduction assay. Infect. Immun. 37, 110-115.
Yason, C.V., Summers, B.A., Schat, K.A., 1987, Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: pathology. Am. J. Vet. Res. 48, 927-938.

How to Cite this Article
Pubmed Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS. 2013; 39(1): 145-154.


Web Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. http://www.alexjvs.com/?mno=44350 [Access: February 08, 2020].


AMA (American Medical Association) Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS. 2013; 39(1): 145-154.



Vancouver/ICMJE Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS. (2013), [cited February 08, 2020]; 39(1): 145-154.



Harvard Style

Maged G. Hemida (2013) Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS, 39 (1), 145-154.



Turabian Style

Maged G. Hemida. 2013. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. Alexandria Journal of Veterinary Sciences, 39 (1), 145-154.



Chicago Style

Maged G. Hemida. "Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates." Alexandria Journal of Veterinary Sciences 39 (2013), 145-154.



MLA (The Modern Language Association) Style

Maged G. Hemida. "Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates." Alexandria Journal of Veterinary Sciences 39.1 (2013), 145-154. Print.



APA (American Psychological Association) Style

Maged G. Hemida (2013) Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. Alexandria Journal of Veterinary Sciences, 39 (1), 145-154.





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