E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 
AJVS. 2013; 39(1): 145-154


Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates

Maged G. Hemida.

Abstract
Rotaviruses are large diverse group of viruses implicated in gastroenteritis in human, different animals and poultry species. They are among the common causes of infantile diarrhea worldwide. The emergence of rotaviruses is a very complicated process. Many rotaviruses can infect several species making their interspecies transmission quiet possible in many cases. The main goal of the current study was to detect the presence of group D avian rotavirus in clinical specimens from chicken farms in both Kafrelsheikh and Gharbia governorates. To achieve our goal we collected cloacal swabs, fecal specimens and pooled organs from several broiler chicken farms from both governorates. In the current study we reported the presence rotavirus in the cloacal swabs and tissue suspensions of pooled organs from chickens suffering from diarrhea. These results had been confirmed by different techniques such as the scanning electron microscope, the immunoelectron microscope, Dot ELISA and the hemagglutination test. Seroconversion of the affected chickens to that virus was documented by the hemagglutination inhibition test. For further confirmation we used a specific oligonucleotdes to amplify a conserved region in group D avian rotavirus VP-6 gene. Our results are showing high degree of identity of the detected viruses to the previously published sequences of group D-avian rotavirus VP-6 gene. This study confirmed for the first time the identification and molecular characterization of group D avian rotavirus in Delta region of Egypt.

Key words: Molecular, detection, group D, avian rotavirus


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Maged G. Hemida
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS. 2013; 39(1): 145-154.


Web Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. https://www.alexjvs.com/?mno=44350 [Access: December 03, 2023].


AMA (American Medical Association) Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS. 2013; 39(1): 145-154.



Vancouver/ICMJE Style

Maged G. Hemida. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS. (2013), [cited December 03, 2023]; 39(1): 145-154.



Harvard Style

Maged G. Hemida (2013) Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. AJVS, 39 (1), 145-154.



Turabian Style

Maged G. Hemida. 2013. Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. Alexandria Journal of Veterinary Sciences, 39 (1), 145-154.



Chicago Style

Maged G. Hemida. "Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates." Alexandria Journal of Veterinary Sciences 39 (2013), 145-154.



MLA (The Modern Language Association) Style

Maged G. Hemida. "Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates." Alexandria Journal of Veterinary Sciences 39.1 (2013), 145-154. Print.



APA (American Psychological Association) Style

Maged G. Hemida (2013) Detection and Molecular Characterization of Group D Avian Rotavirus in Kafrelsheikh and Gharbia Governorates. Alexandria Journal of Veterinary Sciences, 39 (1), 145-154.





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles