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Original Article 


Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem.

Abstract
Four healthy mature bucks of Zaraibi breed were used in this study. Semen samples were collected twice weekly using Electro-ejaculator. Samples with Progressive motility (>80%), total abnormalities (<10%). Five extenders were used, Tris 2.5 % egg yolk extender with 5 ml glycerol as a control, and substitution of whole egg yolk with 4, 6, 8 and10% low density lipoprotein (LDL), respectively. Semen was diluted to 200x106 sperm/ml, and refrigerated (5°C) for three hours, prior to storage in liquid nitrogen (LN) for one week. Sperm progressive motility, life sperm, intact acrosome, plasma membrane integrity and abnormalities were assesd at post-thawing (at 37°C for 30 sec.). The LDL extenders were more effective in preservation of progressive motility, intact acrosome and integrity of the plasma membrane of buck spermatozoa than whole egg yolk extender. Sperm progressive motility, intact acrosome and plasma membrane integrity were much higher (P< 0.05) in the 8 % LDL extender (53.50, 54.64, 54.11) vs. (31.0, 26.31, 37.25) in the control 2.5% egg yolk. It was concluded that LDL 8% in extender improved the freezability of buck spermatozoa.

Key words: LDL,freezing, buck semen


 
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REFERENCES
Aboagla, E.M.E. & Terada, T. 2004. Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology. 62: 1160- 72. [DOI via Crossref]    [Pubmed]   
Aitken, R.J. & McLaughlin, E.A. 2007. Molecular mechanisms of sperm capacitation: progesterone-induced secondary calcium oscillations reflect the attainment of a capacitated state. Soc. Reprod. Fertil. Suppl. 63: 273-293. [Pubmed]   
Al Ahmad, M.Z., Chatagnon, G., Amirat, L., Moussa, M., Tainturier, D., Anton, M. & Fieni, F. 2008. Use of glutamine and low density lipoprotiens isolated from egg yolk to improve buck semen freezing. Reprod. Domestic Anim. 43(4): 429-36. [DOI via Crossref]    [Pubmed]   
Amann, R.P. & Pickett, B. 1987. Principles of cryopreservation and a review of stallion spermatozoa. Equine vet Sci. 7: 145- 173.
Amirat, L., Anton, M., Tainturier, D., Chatagnon, G., Battut, I. & Courtens, J.L. 2005. Modifications of bull spermatozoa induced by three extenders: Biociphos, LDL and Triladyl, before, during and after freezing and thawing. Reprod. 129: 535-543. [DOI via Crossref]    [Pubmed]   
Amirate, L., Tainturier, D., Jeanneau, L., Thorin, C., Gerard, O., Courtens, J.L. & Anton, M. 2004. Bull semen in vitro fertility after cryopreservation using egg yolk extender. Theriogenology. 61(5): 895-907. [DOI via Crossref]   
Andrabi, S.M.H., Ansari, M.S., Ullah, N., Anwar, M., Mehmood, A. & Akhter, S. 2008. Duck egg yolk in extender improves the freezability of buffalo-bull spermatozoa. Anim. Reprod. Sci. 104: 427- 433. [DOI via Crossref]    [Pubmed]   
Anton, M., Martinet, V., Dalgalarrondo, M., Beaumal, V., David-Briand, E. & Rabesona, H. 2003. Chemical and structural charactarization of low density lipoprotiens purified from hen egg yolk. Food Chem. 83: 175-183. [DOI via Crossref]   
Bencharif, D., Amirat, L., Anton, M., Schmitt, E., Desherces, S., Delhomme, G., Langlois, M. L., Barrie`re, P., Larrat, M. & Tainturier, D. 2008. The advantages of LDL (Low Density Lipoproteins) in the cryopreservation of canine semen. Theriogenology. 70: 1478-1488. [DOI via Crossref]    [Pubmed]   
Bergeron, A., Crête, M., Brindle, Y. & Manjunath, P. 2004. Low-density lipoprotein fraction from henÂ’s egg yolk decreases the binding of the major protein of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biol. Reprod. 70: 708-717. [DOI via Crossref]    [Pubmed]   
Bowden, R.M., Ewert, M.A., Lipar, J.L. & Nelson, C.E. 2001. Concentrations of steriod hormones in layers and biopsies of chelonian egg yolks. Gen Comp Endocrinol. 121: 95- 103. [DOI via Crossref]    [Pubmed]   
Celeghini, ECC., Arruda, RP., Andrade, AFC., Nascimento, J., Raphael, CF. & Rodrigues, PHM. 2007. Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim. Reprod. Sci. 100: 1-13.
EL-Sharawy, M.E. 2010. Use of low density lipoproteins and glutamine to improve frozen buffalo bull semen. Doctor of Philosophy (Ph.D.), Kafrelsheikh University.
El-Sharawy, M.E., El-Shamaa, I.S., Ibrahim, M.A.R., Abd El-Razek, I.M. & El-Seify, E.M. 2013. Effect of low density lipoproteins in extender on freezability and fertility of egyptian buffalo bull semen. Anim. Sci. LV: 2285-5750.
Foulkes, JA. 1977. The separation of lipoproteins from egg yolk and effect on the motility and integrity of bovine spermatozoa. J. Reprod. Fertil. 49: 277-284. [DOI via Crossref]    [Pubmed]   
Holt, W. 2000. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology. 53: 47-58. [DOI via Crossref]   
Hu, J.H., Li, O.W., Li, G., Chen, X.Y., Yang, H., Zhang, SS., Wang, L.Q. 2006. The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins. Asian Australas J. Anim. Sci. 19(4): 486-494. [DOI via Crossref]   
Hu, J.H., Qing-Wang, L., Lin-Sen, Z., Zhang-Liang, J., Jun-Hui, A., Li-Qiang, W. & Yong-Hong, J. 2010. The cryoprotective effect of low density lipoprotiens in extenders on bull spermatozoa following freezing-thawing. Anim. Reprod. Sci. 117 (12): 11-17. [DOI via Crossref]    [Pubmed]   
Iritani, A., & Nishikawa, Y. 1963. Studies on the egg-yolk coagulating factor in goat semen. V. Purification of the egg-yolk coagulating enzyme. Jap. J. Anim. Reprod. 8: 118-121. [DOI via Crossref]   
Jiang, Z.L., Li, Q.W., Hu, J.H., Li, W.Y., Zhao, H.W., & Zhang, S.S. 2007. Improvement of the quality of boar cryopreservation semen by supplementing with low density lipoprotein in diluents. Cryobiol. 54:301- 304. [DOI via Crossref]    [Pubmed]   
Johnson, L.A., Weitze, K.F., Fiser, P. and Maxwell, W.M.C. 2000. Storage of boar semen, Anim. Reprod. Sci. 62: 143- 172. [DOI via Crossref]   
Khalifa, E.I., Ahmed, M.E., Abdel-Gawad, A.M. & El-Zelaky, O.A. 2009. Estimation of genetic parameters and some non-genetic factors for litter size at birth and weaning and milk yield traits in Zaraibi goats. Egy. J. Sheep Goat Sci. 4: 55-64.
Khlifaoui M. 2004. Equine semen freezing: three cryoprotectants interest: glutamine, low density lipoproteins from chicken egg yolk and carnitine. PhD Thesis. University of Rennes.
Leboeuf, B., Restall, B. & Salamon, S. 2000. Production and storage of goat semen for artificial insemination.Anim.Reprod.Sci.62:113-141. Le denmat, M., Anton, M. & Beaumal, V. 2000. Characterisation of emulsion properties and of interface composition in oil-in-water emulsions prepared with hen egg yolk, plasma and granules. Food Hydrocolloids. 14: 539-549. [DOI via Crossref]   
Mekasha Gebre, Y. 2007. Reproductive traits in Ethiopian male goat: with special reference to breed and nutrition. PhD Doctoral thesis, Dept. of clinical sciences, Swedish University of Agricultural Sciences.
Manjunath, P., Nauc, V., Bergeron, A. & Menard, M. 2002. Major Proteins of Bovine Seminal Plasma Bind to the Low-Density Lipoprotein Fraction of Hen's Egg Yolk. Biol. Reprod. 67: 1250-1258. [DOI via Crossref]    [Pubmed]   
Memon, A.A., Wahid, H., Rosnina, Y., Goh, Y.M., Ebrahimi, M., Nadia, F.M. & Audrey, G. 2011. Effect of butylated hydroxytoluene on cryopreservation of Boer goat semen in Tris egg yolk extender. Anim. Reprod. Sci. 129: 44- 49. [DOI via Crossref]    [Pubmed]   
Moreno, D., Bencharif, D., Amirat, L., Neira, A., Destrumelle, S. & Tainturier, D. 2013. Preliminary Results: The Advantages of Low-Density Lipoproteins for the Cryopreservation of Equine Semen. J. Equine Vet. Sci. 1-8.
Moussa, M., Martinet, V., Trimeche, A., Tainturier, D. & Anton, M. 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology, 57: 1695-1706. [DOI via Crossref]   
Moustacas, V.S., Zaffalon, F.G., Lagares, M.A., Loaiza-Eccheverri, A.M.,Varago, F.C., Neves, M.M., Heneine, L.G.D., Arruda, R.P. & Henry, M. 2011. Natural, but not lyophilized, low density lypoproteins were an acceptable alternative to egg yolk for cryopreservation of ram sêmen. Theriogenology. 75: 300-307. [DOI via Crossref]    [Pubmed]   
Neves, M.M. 2008. Extrac¸ ão das lipoproteínas de baixa densidade da gema do ovo de Gallus domesticus e sua aplicac¸ ão na criopreservac¸ ãodo sêmen canino, Low density lipoproteins extraction from Gallus domesticus egg yolk and its application in canine sperm cryopreservation. Universida de Federal de Minas Gerais, Minas Gerais, Brazil http://www.bibliotecadigital.ufmg.br/dspace/bitstream/1843/SSLA7UEHL3/1/marianamachadoneves.pdf (Tese).
Pillet, E., Duchamp, G., Batellier, F., Beaumal, V., Anton, M., Desherces, S., Schmitt, S. & Magistrini, M. 2011. Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology. 75: 105-114. [DOI via Crossref]    [Pubmed]   
Purdy, PH. 2006. Areview on goat sperm cryopreservation. small Anim. Res. 63:215- 25.
Revell, S.G. & Mrode, R.A. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci., 36: 77- 86. [DOI via Crossref]   
Shipley, C., Buckrell, B. & Mylne, M. 2007. In: Youngquist, R.S., Threlfall, W.R. (Eds.), Current Therapy in Large Animal Theriogenology., 2nd ed. Saunders-Elsevier, St. Louis, MO, pp. 629-641.
Silva, A.R., Cardoso, R.C.S. & Silva, L.D.M. 2000. Congelac¸ ão de sêmen canino com diferentes concentrac¸ ões de gema de ovo e glicerol em diluidores à base de Tris e água de coco. Cienc.Rural. 30:1021-1025. [DOI via Crossref]   
Silva M.C., Moura, L.C.O., Melo, M.I.V., Mambrini, J.V.M., Nevesd, M.M., Henry, M.R., Snoeck, P.P. 2014. Prolonged post cooling but not pre-cooling equilibrium length improves the viability of ram sperm cryopreserved in an extender containing low-density lipoproteins. Small Rumin. Res. 119: 88-95. [DOI via Crossref]   
Tomas, C., Banch, E. & Moce, E. 2011. Efecto del diluyente, del diluyente, del tiempo y de la altura de congelacion sobre la calidad del semen crioconservado de machos cabrios, in: Asociacion interprofesional para el Desarrollo Agrario (Ed). Proceedings of the XIV Jornadas Sobre Production Animal, Zaragoza. 428- 430. Tonieto, R.A., Goularte, K.L., Gastal, G.D.A., Schiavon, R.S., Deschamps, J.C. & Lucia T.Jr. 2010. Cryoprotectant effect of trehalose and low-density lipoprotein in extenders for frozen ram semen. Small Rumin. Res. 93: 206-209.
Uysal, O. & Bucak, M.N. 2007. Effects of oxidized glutathione, bovine serum albumine cysteine and lycopene on the qualiy of frozen-thawed ram semen. Acta. Vet. Brno. Acta Vet. Brno. 76: 383: 390.
Watson, PF. 1975. The interaction of egg yolk and ram spermatozoa studied with a fluorescent probe. J. Reprod.Fertil.42:105-111. Watson, PF. 1976. Electroejaculation, semen characteristic and semen preservation of the brindled gnu. J. Reprod.Fertil. 47:123-6. [DOI via Crossref]    [Pubmed]   
Watson, P.F. 1981.The effects of cold shock on sperm cell membranes, in: G.J. Morris, A. Clarke (Eds.).
Watson, PF. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60-61:481-492 Witte, T.S., Schafer-Somi, S. 2007. Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Anim. Reprod. Sci. 102: 181-193. Woelders, H., Matthijs, A., Engel, B. 1997. Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiol. 35(2): 93-105. [DOI via Crossref]    [Pubmed]   
Yunsheng, Z. 2008. Effects of LDL and Cholesterol Sulphate on Cryopreservation and Lipid Contents of Goat and Sheep Sper

How to Cite this Article
Pubmed Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS. 2015; 44(1): 93-102. doi:10.5455/ajvs.172299


Web Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. http://www.alexjvs.com/?mno=172299 [Access: August 18, 2021]. doi:10.5455/ajvs.172299


AMA (American Medical Association) Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS. 2015; 44(1): 93-102. doi:10.5455/ajvs.172299



Vancouver/ICMJE Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS. (2015), [cited August 18, 2021]; 44(1): 93-102. doi:10.5455/ajvs.172299



Harvard Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem (2015) Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS, 44 (1), 93-102. doi:10.5455/ajvs.172299



Turabian Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. 2015. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. Alexandria Journal of Veterinary Sciences, 44 (1), 93-102. doi:10.5455/ajvs.172299



Chicago Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. "Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen." Alexandria Journal of Veterinary Sciences 44 (2015), 93-102. doi:10.5455/ajvs.172299



MLA (The Modern Language Association) Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. "Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen." Alexandria Journal of Veterinary Sciences 44.1 (2015), 93-102. Print. doi:10.5455/ajvs.172299



APA (American Psychological Association) Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem (2015) Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. Alexandria Journal of Veterinary Sciences, 44 (1), 93-102. doi:10.5455/ajvs.172299





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