Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem

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Original Article

AJVS. 2015; 44(1): 93-102

doi: 10.5455/ajvs.172299

Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem.

Abstract
Four healthy mature bucks of Zaraibi breed were used in this study. Semen samples were collected twice weekly using Electro-ejaculator. Samples with Progressive motility (>80%), total abnormalities (<10%). Five extenders were used, Tris 2.5 % egg yolk extender with 5 ml glycerol as a control, and substitution of whole egg yolk with 4, 6, 8 and10% low density lipoprotein (LDL), respectively. Semen was diluted to 200x106 sperm/ml, and refrigerated (5°C) for three hours, prior to storage in liquid nitrogen (LN) for one week. Sperm progressive motility, life sperm, intact acrosome, plasma membrane integrity and abnormalities were assesd at post-thawing (at 37°C for 30 sec.). The LDL extenders were more effective in preservation of progressive motility, intact acrosome and integrity of the plasma membrane of buck spermatozoa than whole egg yolk extender. Sperm progressive motility, intact acrosome and plasma membrane integrity were much higher (P< 0.05) in the 8 % LDL extender (53.50, 54.64, 54.11) vs. (31.0, 26.31, 37.25) in the control 2.5% egg yolk. It was concluded that LDL 8% in extender improved the freezability of buck spermatozoa.

Key words: LDL,freezing, buck semen

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Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS. 2015; 44(1): 93-102. doi:10.5455/ajvs.172299

Web Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. http://www.alexjvs.com/?mno=172299 [Access: August 18, 2021]. doi:10.5455/ajvs.172299

AMA (American Medical Association) Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS. 2015; 44(1): 93-102. doi:10.5455/ajvs.172299

Vancouver/ICMJE Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS. (2015), [cited August 18, 2021]; 44(1): 93-102. doi:10.5455/ajvs.172299

Harvard Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem (2015) Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. AJVS, 44 (1), 93-102. doi:10.5455/ajvs.172299

Turabian Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. 2015. Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. Alexandria Journal of Veterinary Sciences, 44 (1), 93-102. doi:10.5455/ajvs.172299

Chicago Style

Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem. "Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen." Alexandria Journal of Veterinary Sciences 44 (2015), 93-102. doi:10.5455/ajvs.172299

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Iman E. El-Bawab, Kamal K. Metwelly, Samia M. Abd El-Rheem (2015) Effect of different concentrations of chicken low density lipoprotein on quality of frozen buck semen. Alexandria Journal of Veterinary Sciences, 44 (1), 93-102. doi:10.5455/ajvs.172299

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