E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Genetic Diversity of Mannheimiahaemolytica strains

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied.

Cited by (1)

Abstract
Mannheimiahaemolyticais the principal bacterial pathogen associated with bovine respiratory disease (BRD). As an opportunistic pathogen, M. haemolyticais also frequently isolated from the respiratory tract of healthy cattle. This study examined the incidence of M. haemolytica using deep nasal swabs from diseased cattle, buffaloes, sheep and goat as well as from their closely contact apparently normal animals (n = 225) and pneumonic lung tissues (n=90) then identification of the isolates by conventional culture method, biochemical and confimed by molecular tecqnique, finally assessment the genentic diversity between the confirmed isolates. Out of 315 samples examined, Mannheimia was isolated in 5% (4/75) ofcattlenasal and lung samples and 3.33%(3/90)of sheep samples. Identification of these isolates were confirmed by polymerase chain reaction (PCR) using species specific primer (PHSSA) derived from serotype specific antigen (ssa) gene. The amplification of genomic DNA obtained was corresponding to the anticipated size of 237bp.PCR was used to screen isolates for virulence outer membrane lipoprotein (gs60), O-sialoglycoproteinendopeptidase (gcp) and antimicrobial resistance (blaROB-1)genes. The virulence genes gs60 and gcp were present in all isolated M. haemolyticaat 329 and 227bp respectively, while antimicrobial resistant blaROB-1 gene was present in only two isolates at 685pb. Finally assessment the genetic differences among M. haemolytica isolates by partial genome sequencing of (gs60) gene blaROB-1 gene.

Key words: Mannheimiahaemolytica strains, bovine, respiratory, diseases


 
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This Article Cited By the following articles

Molecular and Phenotypical Characterization of <i>Mannheimia haemolytica</i> Isolated from Goats in Baghdad Province
AiM 2017; 07(04): 304.

1
 
How to Cite this Article
Pubmed Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. Genetic Diversity of Mannheimiahaemolytica strains. AJVS. 2015; 47(1): 166-174. doi:10.5455/ajvs.200714


Web Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. Genetic Diversity of Mannheimiahaemolytica strains. http://www.alexjvs.com/?mno=200714 [Access: November 27, 2020]. doi:10.5455/ajvs.200714


AMA (American Medical Association) Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. Genetic Diversity of Mannheimiahaemolytica strains. AJVS. 2015; 47(1): 166-174. doi:10.5455/ajvs.200714



Vancouver/ICMJE Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. Genetic Diversity of Mannheimiahaemolytica strains. AJVS. (2015), [cited November 27, 2020]; 47(1): 166-174. doi:10.5455/ajvs.200714



Harvard Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied (2015) Genetic Diversity of Mannheimiahaemolytica strains. AJVS, 47 (1), 166-174. doi:10.5455/ajvs.200714



Turabian Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. 2015. Genetic Diversity of Mannheimiahaemolytica strains. Alexandria Journal of Veterinary Sciences, 47 (1), 166-174. doi:10.5455/ajvs.200714



Chicago Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. "Genetic Diversity of Mannheimiahaemolytica strains." Alexandria Journal of Veterinary Sciences 47 (2015), 166-174. doi:10.5455/ajvs.200714



MLA (The Modern Language Association) Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied. "Genetic Diversity of Mannheimiahaemolytica strains." Alexandria Journal of Veterinary Sciences 47.1 (2015), 166-174. Print. doi:10.5455/ajvs.200714



APA (American Psychological Association) Style

Marwa M. El Dokmak, Samy A. Khalil and Sawsan KH. M. Ebied (2015) Genetic Diversity of Mannheimiahaemolytica strains. Alexandria Journal of Veterinary Sciences, 47 (1), 166-174. doi:10.5455/ajvs.200714





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