E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam.

Abstract
One hundred random samples of cream (45 raw cream, 15 pasteurized cream, 15 whipped creams and 25 cream powders) were collected from dairy shops and supermarkets in Alexandria city for evaluation of chemical quality (fat and acidity %), microbiological status and health hazards. The mean values of fat percent and titratable acidity for examined raw cream samples were 31.5± 0.56 and 0.36± 0.01, while for pasteurized fluid cream were 30.1± 1.08 and 0.40± 0.02, also mean values of fat % and titratable acidity for examined whipped cream were 32.8± 0.89 and 0.37± 0.02 and finally for cream powder samples were 31.6± 0.85 and 0.37± 0.02, respectively. Four isolates of E.coli biochemical identified by VITEK2 Compact, 3 isolate confirmed as E.coli by percent (75%) and one isolate confirmed as citrobacter by percent (25 %), serological identification of 6 isolates of E.coli are –ve poly I, II and III, it mean that all isolates nonpathogenic E.coli. the mean values of total bacterial count of different microorganism isolated from raw cream were 3.01x105±0.7x105, 2.06x105±0.42x105, 5.73x102 ± 0.87x102, 6.11x102±2.06x102 and 7.6x104±1.8x104 and 1.5x104±0.25x104 for total bacterial count, Coliforms, Staphylococcus aureus, enterococci, yeasts and moulds count respectively. The respective mean counts of different microorganism isolated from pasteurized fluid cream were 4.2x102±1.5x102, 1.1x103±0.48x103, 6.5x102 ± 1.5x102, 2.1x102±0.86x102, 2.03x102±1.2x102 and 1.3x103±1.2x103 for total bacterial count, Coliforms, Staphylococcus aureus, enterococci, yeasts and moulds respectively. The mean values of different microorganisms isolated from collected samples of whipped cream were 2.4x104±1.3x104, 1.6x104±0.37x104, 1.1x102±0.5x102, 4.0x10±0.9x10, 4.04x105±0.67x102 and 5.5x102±2.6x102 for total bacterial count, Coliforms, Staphylococcus aureus, enterococci, yeasts and moulds, respectively. The mean values of total bacterial count, Coliforms, yeasts and moulds which isolated from cream powder samples were 1.25x10± 0.25x10, 1.5x10±0.5x10, 1.5x10±0.5x10 and 1.0x10 ±0, respectively. We fail to isolate staphylococcus aureus and enterococci from the cream powder samples.

Key words: Cream, TBC, Coliform


 
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REFERENCES
Alfa-laval 1980. Dairy handbook, Alfa-laval AB, Dairy and food engineering division. Lund, Sweden.
APHA (American Public Health Association) 1992: Standard Methods for Examination of Dairy Products. 16th Ed. American Public Health Association, Washington, DC.
APHA, American Public Health Association 1985: Standard Methods for the Examination of Dairy products.15th Ed., American Public Health Association, New York.
Bailey, W.R., Scott, E.G. 1998.: Diagnostic Microbiology. A textbook for the Isolation and Identification of Pathogenic Microorganisms. The C.V. Mosby Company Saint Louis.
Banwart, G.J. 1998. Basic Food Microbiology 2th Ed. CBS Publishers and Distributors. New Delhi-110002 (India). [DOI via Crossref]   
Benkerroum, N., Bouhal, Y., EI Attar, A., Marhaben, A. 2004: Occurrence of Shiga toxin-producing E. coli 0157:H7 in selected diary and meat products marketed in the city of Rabat, Morocco, J. Food. Prot., 676: 1234-1237.
David H. P. M.S 1988. Microbial identification using the biomérieux vitek ® 2 system. bioMérieux, Inc. Hazelwood, MO, USA.
Doyle M.P., Beuchat L.R., Montville T.J. 2001: Food Microbiology: Fundamentals and Frontiers, 2nded, ASM press, USA. 111-122. [Pubmed]   
Ewing, W.H. 1986. Edwards and Ewing's identification of Enterobacteriaceae. Elsevier Science Publishing Co., Inc., New York, N.Y.; 169-181.
Foster, G. M., Nelson, F.E., Speck, M.L., Doetsch, R.N., Olson, J.C. 1983. Dairy Microbiology. MacMillan & Coltd., London.
Giraffa, G. 2003. Functionality of enterococci in dairy products. International Journal of Food Microbiology, 88(2-3): p. 215-222. [DOI via Crossref]   
Godefay, B., Molla B. 2000. Bacteriological quality of raw milk from four dairy farms and milk collection center in and around Addis Ababa. Berl. Munch. Tierarztl.Wschr. 113: 13-17.
ICMSF, International Committee on Microbiological Specification of Foods 1982: Microorganisms in Foods, their significance and methods of enumeration. 2 nd Ed., Univ. Toronto Press, Toronto, Buffalo, London.
ICMSF, International Committee on Microbiological Specification of Foods 1978: International Commission of Microbiological Specifications for Foods. Microbial ecology of foods, University of Toronto Press, Toronto, Ontario, Canada.
Jorgensen H.J., Mork T., Hogasen, H.R., Rorvik, L.M. 2005: J. Appl. Microbiol., 99 (1): 158-166. [DOI via Crossref]    [Pubmed]   
Kamat, M.Y., Sulebele, G., Nirupama, Sabnis 1991: A comparative evaluation of media for enumeration of enterotoxigenic Staphylococci by selective enrichment technique. J. Food Sci. Technol., India, 28 (6): 381-383.
Marth, E.H., Steel, J.L. 2001. Applied Dairy microbiology. 2nd Ed., Marcel Dekker, Inc., New York, USA.
Mossel, D.A.A. 1982. Microbiology of Foods. 3rd Ed., the Univ. of Utrecht, the Netherlands ISBN. [PMC Free Fulltext]   
Roushdy, I.M., Ehrmann, M.A., Vogel, R.F. 1997. Molecular identification and characterization of halo-tolerant lactic acid bacteria isolated from soft pickled Damietta cheese. Adv. Food Sci. 20 (1/2): 40-45.
Thatcher, F.S., Clark, D.S. 1982. Microorganisms in Foods. 2nd Ed. Toronto Buffalo, London. [PMC Free Fulltext]   
Tsen, H.Y., Yian, L.Z., Chi, W. R. 1998. Use of multiplex PCR system for the simultaneous detection of heat labile toxin I and heat stable toxin II genes of enterotoxigenic Escherichia coli in skim milk and porcine stool. J. Food Prot, 141-145. [Pubmed]   
World Health organization Food and Agriculture Organization Codex Alimentarius 2011: standards of cream powder.

How to Cite this Article
Pubmed Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. AJVS. 2015; 47(1): 209-215. doi:10.5455/ajvs.202990


Web Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. http://www.alexjvs.com/?mno=202990 [Access: November 28, 2020]. doi:10.5455/ajvs.202990


AMA (American Medical Association) Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. AJVS. 2015; 47(1): 209-215. doi:10.5455/ajvs.202990



Vancouver/ICMJE Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. AJVS. (2015), [cited November 28, 2020]; 47(1): 209-215. doi:10.5455/ajvs.202990



Harvard Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam (2015) Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. AJVS, 47 (1), 209-215. doi:10.5455/ajvs.202990



Turabian Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. 2015. Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. Alexandria Journal of Veterinary Sciences, 47 (1), 209-215. doi:10.5455/ajvs.202990



Chicago Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. "Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City." Alexandria Journal of Veterinary Sciences 47 (2015), 209-215. doi:10.5455/ajvs.202990



MLA (The Modern Language Association) Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam. "Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City." Alexandria Journal of Veterinary Sciences 47.1 (2015), 209-215. Print. doi:10.5455/ajvs.202990



APA (American Psychological Association) Style

Ashraf M. Nazem, Abbas A. Ahamed, Mohamed M. Essam (2015) Microbiological Value and Healthy Hazard Associated with Cream Sold in Local Markets at Alexandria City. Alexandria Journal of Veterinary Sciences, 47 (1), 209-215. doi:10.5455/ajvs.202990





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