E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela.

Abstract
This study used Random Amplified Polymorphic DNA technique (RAPD) to characterize Tilapia species and their hybrid (O. niloticus, O. aureus, S. galilaeus, T. zillii and Hybrid (O. niloticus x O. aureus)). Three samples of each species were collected from River Nile. DNA of the fish samples were extracted using DNA extraction kit. RAPD analysis was carried out using eleven RAPD primers, while only eight primers were given detectable bands. A total of 186 scorable bands obtained by RAPD-PCR technique across four tilapia species and hybrid genomes with 166 polymorphic bands. The similarity indices were high among tilapia species, science it ranged from 0.920 between O. aureus and O. niloticus, to 0.907 between hybrid (O. niloticus x O. aureus) and S. galilaeus. Therefore the phylogenetic relationships among the four tilapia species and hybrid were clearly resolved in dendrograms. They clustered in five branches: the first one includes O. niloticus, the second branch includes O. aureus, the third branch includes S. galilaeus, the fourth branch includes hybrid (O. niloticus x O. aureus) and the fifth branch includes T. zillii. This study concluded that, the molecular patterns showed by RAPD bands, represent specific bands and apparently are suitable tool to distinguish among tilapia species and hybrid. The results clearly revealed that the four tilapia and hybrid are distinctive species.

Key words: Genetic diversity, RAPD, Phylogenetic relationships and Tilapia


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Walaa S.H. Abd El Naby
Articles by Sherif M. Nasr and Ramy M. Shourbela
on Google
on Google Scholar


REFERENCES
Abd Elkader, H. A. M., Abd Elhamed, Z. G., Mahrous, K. F. 2013. Genetic Diversity among Three Species of Tilapia in Egypt Detected by Random Amplified Polymorphic DNA Marker. J. Biol. Sci. 7 (2): 57-64.
Ahmed, M. M. M., Ali, B. A., El-Zaeem, S. 2004. Application of RAPD markers in fish: part-I some genera (Tilapia, Sarotherodon and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of tilapia. Int. J. Biotechol. 6: 86-93. [DOI via Crossref]   
Baradakci, F., Skibinski, D. O. F. 1994. Application of the RAPD technique in Tilapia fish: species and subspecies identification. Hered. 73: 117-123. [DOI via Crossref]   
Bernardi, G., Talley, D. 2000. Genetic evidence for limited dispersal in the coastal California killifish, Fundulus parvipinnis; J. Exp. Mar. Biol. Ecol. 255: 187–199. [DOI via Crossref]   
Cagigas, M. E., Vazquez, E., Blanco, G., Sánchez, J. A. 1999. Combined assessment of genetic variability in populations of brown trout (Salmo trutta L.) based on allozymes, microsatellites and RAPD markers; Mar. Biotechnol. 1: 286–296. [DOI via Crossref]   
Chen, H. Leibenguth, F. 1995. Studies on multilocus fingerprints, RAPD markers, and mitochondrial DNA of a gynogenetic fish Carassius auratus gibelio. Biochem. Genet. 3: 297–306. [DOI via Crossref]   
Corley-Smith, G. E., Lim, C.J., Brandhorst, B. P. 1996. Production of androgenetic zebra fish Danio rerio. Genetics. 142: 265–276.
Das, P., Prasad, H., Meher, P. K., Barat, A. K., jana, R. K. 2005. Evaluation of genetic relationship among six Labeo species using Random Amplified Polymophic DNA (RAPD). Aqucult. Res. 36: 564-569. [DOI via Crossref]   
Hadrys, H., Balick, M., Schierwater, B. 1992. Application of random amplified polymorphic DNA (RAPD) in molecular ecology. Mo. Eco. 1: 55-63. [DOI via Crossref]   
Holsinger, K.E., Lewis, P.O. Dey, D.K. 2002. A Bayesian approach to inferring population structure from dominant markers. Mol. Ecol. 11: 1157–1164. [DOI via Crossref]    [Pubmed]   
Kazianis, S., Morizot, D. C., McEntire, B. B., Nairn, R.S., Borowsky, R. L. 1996. Genetic mapping in Xiphophorus hybrid fish: assignment of 4 3 AP-PCR/RAPD and isozyme markers to multipoint linkage groups. Genome Res. 4: 280-289. [DOI via Crossref]   
Levin, I., Crittenden, L. B., Dodgson, J. B. 1993. Genetic map of the chicken Z-chromosome using random amplified polymorphic DNA (RAPD) markers; Genomics 16 224–230. [DOI via Crossref]    [Pubmed]   
Lu, R., Rank, G. H. 1996. Use of RAPD analysis to estimate population genetic parameters in the Alfalfa leaf cutting bee, Megachite rotundate. Genome. 39: 655-663 [DOI via Crossref]    [Pubmed]   
Lynch, M. 1990. The similarity index and DNA fingerprinting. Mol. Biol. Evol., 7, 478-484. [Pubmed]   
Popma, T.J., Lovshin, L.L., 1996. "World prospects for commercial production of tilapia". Research and Development Series 41. International Center for Aquatic Environment, Department of Fisheries and Allied Aquaculture, Auburn University,Alabama, USA. [PMC Free Fulltext]   
Postlethwait, J. H., Johnson, S. L., Midson, C. N., Talbot, W. S., Gates, M. 1994. A Genetic linkage map for the Zebra fish. Sci. 264: 699-705 [DOI via Crossref]   
Rashed, M. A., Saad, Y. M., Atta, A. H., Ahmed, N. E. 2011. Reconstruction of phylogenetic relations among four tilapia species. World Appl. Sci. J. 14: 456-462.
Rashed, M. A., Saad, Y. M., EL – Seoudy, A. A., Ibrahim, M. M. 2009. Gene flow in some Oreochromis niloticus populations based on SSR linked markers to MHC loci class I. J. Boil. Chem. Environmen. Sci., 4 (1): 319 - 331.
Rashed, M. A, Saad, Y. M., Ibrahim, M. M., EL – Seoudy, A. A. 2008. Genetic structure of Natural Egyptian Oreochromis niloticus evaluated from dominant DNA markers. Global Vet. 2(2): 87 - 91.
Saad, Y.M., Rashed, M. A., Atta, A. H., Ahmed, N. E. 2012. Genetic Diversity among Some Tilapia Species Based on ISSR Markers. Life Sci. J. 9(4): 4841-4846.
Saad, Y.M. Rashed, M. A., El-Deep, S. I., El-Gamal, A. A., Saiid, M. 2002. Molecular genetic markersand phylogenetic relations for some tilapia species. J. Arab Biol. Cairo. 18 (A): 27-44.
Sneath, P. H. A., Sokal, R.R. 1973. Numerical Taxonomy. The Principles and Practice of Numerical Classification. W.H. Freeman and Co., San Francisco: 573
Soufy. H., Laila, A. M., Iman, M. K. A. 2009. RAPD-PCR for DNA-fingerprinting of Egyptian tilapia. New York Sci. J. 2:1554-0200.
Statistica. Ver. 5, 1995. Stat Soft, Inc., Tulsa, Ok 74104, USA
Strauss, R. E., Bond. C. E. 1990. Taxonomic methods: morphology. In: Schreck CB, Moyle PB (eds) Methods for fish biology. American Fisheries Society, Maryland, pp 109–140
Trewavas, E., 1982. Tilapias: taxonomy and speciation, in: Pullin, R.S.V., Lowe McConnell, R.H. (Eds.), The biology and culture of tilapias. Manila Philippines: ICLARM Conference Proceedings Number 7, pp. 3-13.
Trewavas, E., 1983. Tilapiine fishes of the genera Sarotherodon, Oreochromis and Danakilia. Itahaca, NY: University Press. [Pubmed]   
Upadhya, S. K. Jun, W. Yong-Quan, S. Shao-Xiong, D., Chaturvedi, S. 2006. Genetic diversity of yellow grouper (Epinephelus awoara) determined by random amplified polymorphic DNA (RAPD) analysis. Fish. Bull. 104: 638-642.
Welsh, J., Petersen, C. McClelland, M. 1991. Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping; Nucleic Acids Res. 19: 303–306. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Welsh, J., McClelland, M. 1990. Fingerprinting genome using PCR with arbitrary primers. Nucl. Acids Res. 18: 7213–7218. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Williams, J. G. K., Kublik, A. R., Livak, K. J., Rafalski, J. A. Tingey, S.V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531–6535 [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Williams, S. G. K., Hanafey, M. K., Rafalski, J. A. Tingey, S. V. 1993. Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol. 218: 704-740. [DOI via Crossref]   
Yoon, J. M., Kim, G.W. 2001. Randomly amplified polymorphic DNA-polymerase chain reaction analysis of two different populations of cultured Korean catfish Silurus asotus. J. Biosci. 26: 641–647. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. AJVS. 2015; 47(1): 201-208. doi:10.5455/ajvs.207750


Web Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. http://www.alexjvs.com/?mno=207750 [Access: November 27, 2020]. doi:10.5455/ajvs.207750


AMA (American Medical Association) Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. AJVS. 2015; 47(1): 201-208. doi:10.5455/ajvs.207750



Vancouver/ICMJE Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. AJVS. (2015), [cited November 27, 2020]; 47(1): 201-208. doi:10.5455/ajvs.207750



Harvard Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela (2015) Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. AJVS, 47 (1), 201-208. doi:10.5455/ajvs.207750



Turabian Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. 2015. Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. Alexandria Journal of Veterinary Sciences, 47 (1), 201-208. doi:10.5455/ajvs.207750



Chicago Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. "Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis." Alexandria Journal of Veterinary Sciences 47 (2015), 201-208. doi:10.5455/ajvs.207750



MLA (The Modern Language Association) Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela. "Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis." Alexandria Journal of Veterinary Sciences 47.1 (2015), 201-208. Print. doi:10.5455/ajvs.207750



APA (American Psychological Association) Style

Walaa S.H. Abd El Naby, Sherif M. Nasr and Ramy M. Shourbela (2015) Genetic Variation among Four Species of Tilapia and Their Hybrid in Egypt Using Random Amplified Polymorphic DNA (RAPD) Analysis. Alexandria Journal of Veterinary Sciences, 47 (1), 201-208. doi:10.5455/ajvs.207750





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]