E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa

Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel.

SUPPLEMENTARY FILES :

  • 31-1493031795-adt-1.pdf ()
  • Abstract
    In vitro fertilization (IVF) is a necessary procedure in the in vitro production of embryo. Spermatozoa need to undergo capacitation to be able to perform the acrosome reaction which is required for zona pellucida penetration. Different additives are used to aid in that process, such as caffeine and theophylline. In this study, the effects of IVF media supplementation with D- penicillamine, Hypotaurine and epinephrine (PHE) on fertilization and cleavage rates were assessed. Cumulus oocyte complexes (COCs) containing a compact cumulus cells and normal homogenous cytoplasm were selected. In vitro maturation (IVM) was performed in TCM199 supplied with hormones overnight in a culture conditions of 38.5C and 5% CO2 in air. After 20-22 hr. of maturation, oocytes were randomly assigned to five groups of sperm-TALP media (control and four different PHE concentrations) then inseminated. The fertilization rate of the expected embryos were assessed after 22 hr. after staining with aceto-orcin 1% or cultured for 48 hr. to estimate the cleavage rate. The current data revealed that, 25 l/ml PHE significantly (P< 0.05) improved fertilization (55.93 vs 36.96%) and cleavage (46.15 vs 22.22%) rates when compared to control.

    Key words: D- Penecillamine, Hypotaurine, Epinephrine, In vitro fertilization, Buffalo


     
    ARTICLE TOOLS
    Abstract
    PDF Fulltext
    How to cite this articleHow to cite this article
    Citation Tools
    Related Records
     Articles by Ahmed M. El-Ruby
    Articles by Mamdouh S. Hussein
    Articles by Wael Bedier
    Articles by Abdelmonem M. Montaser
    Articles by Magdy R. Badr
    Articles by Abdelraouf O. Hegab
    Articles by Samy M. Zaabel
    on Google
    on Google Scholar


    How to Cite this Article
    Pubmed Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS. 2017; 53(2): 6-10. doi:10.5455/ajvs.264914


    Web Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. http://www.alexjvs.com/?mno=264914 [Access: August 17, 2021]. doi:10.5455/ajvs.264914


    AMA (American Medical Association) Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS. 2017; 53(2): 6-10. doi:10.5455/ajvs.264914



    Vancouver/ICMJE Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS. (2017), [cited August 17, 2021]; 53(2): 6-10. doi:10.5455/ajvs.264914



    Harvard Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel (2017) Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. AJVS, 53 (2), 6-10. doi:10.5455/ajvs.264914



    Turabian Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. 2017. Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. Alexandria Journal of Veterinary Sciences, 53 (2), 6-10. doi:10.5455/ajvs.264914



    Chicago Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. "Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa." Alexandria Journal of Veterinary Sciences 53 (2017), 6-10. doi:10.5455/ajvs.264914



    MLA (The Modern Language Association) Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel. "Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa." Alexandria Journal of Veterinary Sciences 53.2 (2017), 6-10. Print. doi:10.5455/ajvs.264914



    APA (American Psychological Association) Style

    Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel (2017) Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa. Alexandria Journal of Veterinary Sciences, 53 (2), 6-10. doi:10.5455/ajvs.264914





    Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]