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2026, Vol: 88
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Original Article
Online Published: 20 Nov 2025
 


Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa

Mariam Takla, Sayed A. Hattab, Wael Mb. Noseir, Mostafa S. Fadel, Amany M. Taha, Reham Waheeb.


Abstract
Novel protectants are needed for optimizing freezability of buffalo bull spermatozoa in order to achieve higher fertility outcome. For this purpose, the present study investigated the effect of adding Tribulus Terrestris (TT) and Lepidium Sativum (LS) extract to semen extender on post-thawed quality of buffalo (Bubalus bubalis) bull spermatozoa. Six mature Egyptian buffaloes were used for semen collection, following initial evaluation, ejaculates were diluted with Tris-egg yolk- extender enriched with five concentrations of TT or LS extract: 0 extract (control), 5µg/ml, 20µg/ml, 50µg/ml, and 100µg/ml). The extended semen was cooled to 5°C then packaged into 0.25 ml straws, frozen on liquid nitrogen (LN) vapor and stored in LN tank. After few days, frozen straws were thawed at 37°C for 30 seconds for spermatological characteristic evaluation. Additionally, malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured. Results showed that post-thawed motility, viability, plasma/acrosome membrane integrity, and mitochondrial activity were significantly improved (P <0.05) when 20µg/ml of TT or 5µg/ml of LS was included in the extender compared to control. Moreover, these doses of TT or LS reduced lipid peroxidation (lower MDA levels) and boosted TAC level, but DNA integrity did not show a significant change. TT at 100µg/ml adversely affected these parameters. In conclusion, TT (20µg/ml) or (LS 5µg/ml) extract as semen additives provided protection against cryodamage and hence improved post-thawing sperm characteristics, which might enhance its fertility potential in AI.

Key words: Antioxidants, Tribulus Terrestris, buffalo bull, semen, Lepidium Sativum, cryopreservation.


 
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How to Cite this Article
Pubmed Style

Takla M, Hattab SA, Noseir WM, Fadel MS, Taha AM, Waheeb R. Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. AJVS. 2026; 88(0): 215-223. doi:10.5455/ajvs.291423

Web Style

Takla M, Hattab SA, Noseir WM, Fadel MS, Taha AM, Waheeb R. Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. https://www.alexjvs.com/?mno=291423 [Access: February 04, 2026]. doi:10.5455/ajvs.291423

AMA (American Medical Association) Style

Takla M, Hattab SA, Noseir WM, Fadel MS, Taha AM, Waheeb R. Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. AJVS. 2026; 88(0): 215-223. doi:10.5455/ajvs.291423


Vancouver/ICMJE Style

Takla M, Hattab SA, Noseir WM, Fadel MS, Taha AM, Waheeb R. Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. AJVS. (2026), [cited February 04, 2026]; 88(0): 215-223. doi:10.5455/ajvs.291423


Harvard Style

Takla, M., Hattab, . S. A., Noseir, . W. M., Fadel, . M. S., Taha, . A. M. & Waheeb, . R. (2026) Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. AJVS, 88 (0), 215-223. doi:10.5455/ajvs.291423


Turabian Style

Takla, Mariam, Sayed A. Hattab, Wael Mb. Noseir, Mostafa S. Fadel, Amany M. Taha, and Reham Waheeb. 2026. Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. Alexandria Journal of Veterinary Sciences, 88 (0), 215-223. doi:10.5455/ajvs.291423


Chicago Style

Takla, Mariam, Sayed A. Hattab, Wael Mb. Noseir, Mostafa S. Fadel, Amany M. Taha, and Reham Waheeb. "Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa." Alexandria Journal of Veterinary Sciences 88 (2026), 215-223. doi:10.5455/ajvs.291423


MLA (The Modern Language Association) Style

Takla, Mariam, Sayed A. Hattab, Wael Mb. Noseir, Mostafa S. Fadel, Amany M. Taha, and Reham Waheeb. "Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa." Alexandria Journal of Veterinary Sciences 88.0 (2026), 215-223. Print. doi:10.5455/ajvs.291423


APA (American Psychological Association) Style

Takla, M., Hattab, . S. A., Noseir, . W. M., Fadel, . M. S., Taha, . A. M. & Waheeb, . R. (2026) Impact of Supplementing Freezing Extender with Tribulus Terrestris or Lepidium Sativum Plant Extract on Post-Thawed Quality of Buffalo Bull Spermatozoa. Alexandria Journal of Veterinary Sciences, 88 (0), 215-223. doi:10.5455/ajvs.291423




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Egypt.

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