E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 91
91Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 24 Jul 2026
 


Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases

Nada El Mallah, Amal Eid, Adel Abdelkhalek, Mukhtar El Tarabily.


Abstract
Since the first report of avian reovirus (ARV) infection in Egypt in 1983, the virus has become endemic, with a significant negative impact on the poultry industry. This study was conducted to identify ARV in suspected flocks. For this purpose, eighteen chicken broiler flocks were investigated (October 2025 to May 2026). Initially, real-time reverse transcriptase polymerase chain reaction (rRT-PCR) was applied as the primary diagnostic method for viral detection. However, due to the low number of positive cases detected, enzyme-linked immunosorbent assay (ELISA) was subsequently used to further evaluate the infection status. Clinically affected flocks exhibited runting–stunting syndrome (RSS)/malabsorption syndrome, besides viral arthritis/tenosynovitis (VA) in ten flocks. rRT-PCR revealed the presence of ARV RNA in 6 flocks, but 10 flocks were positive for seroconversion using ELISA. Analysis of the results obtained from ARV-seromolecular diagnosis revealed several variable patterns; six flocks are positive for rRT-PCR, but ten flocks were positive for ELISA assay, including nine flocks with CV% ranging from 6.7% to 170.2%, indicating possible field infection. While four flocks evidenced positive for both rRT-PCR and ELISA. It could be concluded that the schematic diagnosis from day one of age with rRT-PCR on fecal swabs and/or tissue samples with paired serological monitoring to evaluate maternal immunity and confirm ARV field virus exposure as well as any other contributing agent.

Key words: avian reovirus; enzyme-linked immunosorbent assay; malabsorption syndrome; chickens.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Nada El Mallah
Articles by Amal Eid
Articles by Adel Abdelkhalek
Articles by Mukhtar El Tarabily
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Mallah NE, Eid A, Abdelkhalek A, Tarabily ME. Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. AJVS. 2026; 91(0): 41-49. doi:10.5455/ajvs.322738

Web Style

Mallah NE, Eid A, Abdelkhalek A, Tarabily ME. Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. https://www.alexjvs.com/?mno=322738 [Access: October 18, 2026]. doi:10.5455/ajvs.322738

AMA (American Medical Association) Style

Mallah NE, Eid A, Abdelkhalek A, Tarabily ME. Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. AJVS. 2026; 91(0): 41-49. doi:10.5455/ajvs.322738


Vancouver/ICMJE Style

Mallah NE, Eid A, Abdelkhalek A, Tarabily ME. Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. AJVS. (2026), [cited October 18, 2026]; 91(0): 41-49. doi:10.5455/ajvs.322738


Harvard Style

Mallah, N. E., Eid, . A., Abdelkhalek, . A. & Tarabily, . M. E. (2026) Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. AJVS, 91 (0), 41-49. doi:10.5455/ajvs.322738


Turabian Style

Mallah, Nada El, Amal Eid, Adel Abdelkhalek, and Mukhtar El Tarabily. 2026. Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. Alexandria Journal of Veterinary Sciences, 91 (0), 41-49. doi:10.5455/ajvs.322738


Chicago Style

Mallah, Nada El, Amal Eid, Adel Abdelkhalek, and Mukhtar El Tarabily. "Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases." Alexandria Journal of Veterinary Sciences 91 (2026), 41-49. doi:10.5455/ajvs.322738


MLA (The Modern Language Association) Style

Mallah, Nada El, Amal Eid, Adel Abdelkhalek, and Mukhtar El Tarabily. "Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases." Alexandria Journal of Veterinary Sciences 91.0 (2026), 41-49. Print. doi:10.5455/ajvs.322738


APA (American Psychological Association) Style

Mallah, N. E., Eid, . A., Abdelkhalek, . A. & Tarabily, . M. E. (2026) Seromolecular Diagnosis of Avian Reovirus in Broiler Chickens: Analytical Interpretation of Field Cases. Alexandria Journal of Veterinary Sciences, 91 (0), 41-49. doi:10.5455/ajvs.322738




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    Author LoginReviewer LoginAbout PublisherEditorial PoliciesEditorial Review PolicyPeer Review PolicyEditorial & Peer Review ProcessAuthor's Rights and ObligationsPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information