E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 91
91Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 29 Jul 2026
 


Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate

Hadeer H. Mortada, Lamiaa G. Mostafa, Samy A. Khalil.


Abstract
Foodborne diseases (FBDs) remain a significant public health challenge in Egypt, particularly concerning resilient spore-forming pathogens like Clostridium perfringens. This study investigated the prevalence and molecular profile of C. perfringens in 120 canned fish samples (60 mackerel; 60 sardine) retailed in Alexandria governorate between September 2024 and February 2025. Biochemical identification revealed a uniform prevalence of 6.67% (4/60) for both mackerel and sardine samples. Molecular surveillance using PCR identified the species-specific cpa gene in eight isolates and the enterotoxin-encoding cpe gene in six isolates (four mackerel; two sardine). Sequence analysis of the cpe amplicons showed high homology (95.07%–97.65%) to the C. perfringens strain A1 CPE enterotoxin gene. These findings indicate a breach of the Egyptian Standard No. 287/2005, which mandates the total absence of anaerobic spore-formers in canned seafood. The high genetic identity among isolates suggests potential endemic contamination within local manufacturing or handling environments. This study underscores the necessity for rigorous molecular monitoring to ensure the safety of ready-to-eat canned products.

Key words: Clostridium perfringens; cpe gene; Canned fish; Alexandria; PCR, gene sequencing


 
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How to Cite this Article
Pubmed Style

Mortada HH, Mostafa LG, Khalil SA. Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. AJVS. 2026; 91(0): 127-136. doi:10.5455/ajvs.324272

Web Style

Mortada HH, Mostafa LG, Khalil SA. Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. https://www.alexjvs.com/?mno=324272 [Access: October 18, 2026]. doi:10.5455/ajvs.324272

AMA (American Medical Association) Style

Mortada HH, Mostafa LG, Khalil SA. Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. AJVS. 2026; 91(0): 127-136. doi:10.5455/ajvs.324272


Vancouver/ICMJE Style

Mortada HH, Mostafa LG, Khalil SA. Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. AJVS. (2026), [cited October 18, 2026]; 91(0): 127-136. doi:10.5455/ajvs.324272


Harvard Style

Mortada, H. H., Mostafa, . L. G. & Khalil, . S. A. (2026) Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. AJVS, 91 (0), 127-136. doi:10.5455/ajvs.324272


Turabian Style

Mortada, Hadeer H., Lamiaa G. Mostafa, and Samy A. Khalil. 2026. Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. Alexandria Journal of Veterinary Sciences, 91 (0), 127-136. doi:10.5455/ajvs.324272


Chicago Style

Mortada, Hadeer H., Lamiaa G. Mostafa, and Samy A. Khalil. "Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate." Alexandria Journal of Veterinary Sciences 91 (2026), 127-136. doi:10.5455/ajvs.324272


MLA (The Modern Language Association) Style

Mortada, Hadeer H., Lamiaa G. Mostafa, and Samy A. Khalil. "Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate." Alexandria Journal of Veterinary Sciences 91.0 (2026), 127-136. Print. doi:10.5455/ajvs.324272


APA (American Psychological Association) Style

Mortada, H. H., Mostafa, . L. G. & Khalil, . S. A. (2026) Molecular Detection of Certain Enterotoxins of Clostridium Perfringens isolated from Canned Fish Retailed in Alexandria Governorate. Alexandria Journal of Veterinary Sciences, 91 (0), 127-136. doi:10.5455/ajvs.324272




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Alexandria University
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    Abees 10th district, Alexandria
    Egypt.

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