E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid

Hanan Saad El-Samahy.

Abstract
This work aimed to investigate experimentally the effect of lipoic acid as super-antioxidant on the balance between immune and antioxidant status of birds fed aflatoxicated diet as well as histological alterations of immune organs including spleen, thymus, bursa of fabricious and liver. One hundred and twenty-one-day old chicks, fed basal diet till 8th day of age then divided into 4 groups (30 birds/group), each group subdivided into 3 replicates (10 birds/replicate). Group 1: aflatoxicated chickens (125 g/kg diet). Group 2: aflatoxicated lipoic treated (300 mg/kg diet), Group 3: lipoic treated. Group 4: non-treated (control). The blood samples were collected at 14th day post aflatoxicosis and at the end of experiment, then the serum was separated for HI and different biochemical tests. At the end of experiment, four birds from each group were sacrificed and immune organs (thymus, spleen, bursa of fabricious and liver) were collected for histopathology. Livers were also collected for measurement of apoptotic gene expression Caspase (Cystinyle Aspartate Protease), MDA (Malano-Di-Aldehyde) and TAC (Total Antioxidant Capacity). The results showed the addition of lipoic acid into the aflatoxicated broiler ration improved the antibody titer against Newcastle Disease Virus NDV, hepatic apoptotic gene expression, TAC, and the level of different biochemical parameters (protein profile, lipid profile, bilirubin, total calcium and alkaline phosphatase). It was alleviated the toxic effect of aflatoxin on the histological structure of liver, and immune organs. Therefore, lipoic acid could be implicated as a dependent approach in the control of aflatoxicosis in broiler chickens.
Keywords: Aflatoxin, broiler chickens, histopathology, immunity, lipoic acid.

Key words: Aflatoxin, broiler chickens, histopathology, immunity, lipoic acid.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hanan Saad El-Samahy
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Hanan Saad El-Samahy. Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. AJVS. 2021; 69(2): 85-97. doi:10.5455/ajvs.72701


Web Style

Hanan Saad El-Samahy. Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. http://www.alexjvs.com/?mno=72701 [Access: August 17, 2021]. doi:10.5455/ajvs.72701


AMA (American Medical Association) Style

Hanan Saad El-Samahy. Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. AJVS. 2021; 69(2): 85-97. doi:10.5455/ajvs.72701



Vancouver/ICMJE Style

Hanan Saad El-Samahy. Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. AJVS. (2021), [cited August 17, 2021]; 69(2): 85-97. doi:10.5455/ajvs.72701



Harvard Style

Hanan Saad El-Samahy (2021) Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. AJVS, 69 (2), 85-97. doi:10.5455/ajvs.72701



Turabian Style

Hanan Saad El-Samahy. 2021. Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. Alexandria Journal of Veterinary Sciences, 69 (2), 85-97. doi:10.5455/ajvs.72701



Chicago Style

Hanan Saad El-Samahy. "Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid." Alexandria Journal of Veterinary Sciences 69 (2021), 85-97. doi:10.5455/ajvs.72701



MLA (The Modern Language Association) Style

Hanan Saad El-Samahy. "Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid." Alexandria Journal of Veterinary Sciences 69.2 (2021), 85-97. Print. doi:10.5455/ajvs.72701



APA (American Psychological Association) Style

Hanan Saad El-Samahy (2021) Gene Expression, Immunological and Oxidative Indices of Aflatoxicated Broiler Chickens Supplemented with Alpha-Lipoic Acid. Alexandria Journal of Veterinary Sciences, 69 (2), 85-97. doi:10.5455/ajvs.72701





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]