E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate

Nahla A. Ebied, Mostafa S. Abdou, Mona E. Abass.

Abstract
Chicken eggs are important as food to humans. The present study was conducted in Kafrelsheikh governorate, Egypt. A total of 250 samples (each sample contains 3 eggs) were purchased from different retail stores (conventional and organic, 125samples for each). These samples were subjected to microbiological examination for assessment of the sanitary quality and detection of incidence, serological and molecular characterization of salmonella, and subjected to chemical examination to detect the incidence of 2 antibiotic residues (oxytetracycline and tylosin) in both types of egg. Aerobic plate count showed mean values of 8.0102 4.610 cfu/ml for conventional eggs and 6.2102 3.210 cfu/ml for organic eggs, the coliform count showed a mean value of 4.6102 3.210 cfu /ml and 3.7102 2.6 10 cfu/ml for conventional and organic eggs respectively. Staphylococcus aureus count showed a mean value of 3.11023.1 10 cfu/ml for conventional eggs and 2.3102 2.7 10 cfu/ml for organic egg. Mold and yeast count showed a mean value of 7.310 3.810 cfu/ml and 4.210 1.8 10 cfu/ml for conventional and organic eggs respectively. Salmonella was detected in 4 samples (1.6 % from total samples). All of them were only isolated from conventional eggs with a percentage of 3.2%. Serotype identification of four isolates was S. Kentucky. Salmonella Species were not isolated from organic egg contents. Multiplex PCR detected 3 virulence genes (hila, spvC, and stn) (100% of Salmonella isolates). No positive samples for microbial inhibitor test and no antibiotic residues in organic eggs and 4 conventional egg samples were detected positive for microbial inhibitor test with bacillus subtilis, which confirmed by HPLC for detection of 2 antibiotic residues (oxytetracycline and tylosin) and results revealed that 2 out of 4 positive inhibitor test samples were positive for oxytetracycline residues (317 g /gm and 382 g /gm) respectively, and 3 out of the same 4 samples were positive for tylosin residues (8.6 g /gm, 48 g /gm, and 18.5 g /gm) respectively, and all positive samples exceeded the maximal residues limit (MRL 0.2 g /gm) for both oxytetracycline and tylosin residues. The rate of detection of oxytetracycline and tylosin residues was (1.60%) and (2.4%) respectively from the conventional egg. Public health and economic importance for the isolated microorganisms and antibiotic residues were investigated. Results revealed that organic egg is safer than the conventional one.

Key words: Microbial quality, Salmonellae, multiplex PCR, Bacillus subtilis antibiotic residues, organic and conventional table eggs.


 
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How to Cite this Article
Pubmed Style

Ebied NA, Abdou MS, Abass ME. Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. AJVS. 2021; 69(2): 98-112. doi:10.5455/ajvs.83322


Web Style

Ebied NA, Abdou MS, Abass ME. Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. http://www.alexjvs.com/?mno=83322 [Access: August 17, 2021]. doi:10.5455/ajvs.83322


AMA (American Medical Association) Style

Ebied NA, Abdou MS, Abass ME. Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. AJVS. 2021; 69(2): 98-112. doi:10.5455/ajvs.83322



Vancouver/ICMJE Style

Ebied NA, Abdou MS, Abass ME. Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. AJVS. (2021), [cited August 17, 2021]; 69(2): 98-112. doi:10.5455/ajvs.83322



Harvard Style

Ebied, N. A., Abdou, . M. S. & Abass, . M. E. (2021) Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. AJVS, 69 (2), 98-112. doi:10.5455/ajvs.83322



Turabian Style

Ebied, Nahla A., Mostafa S. Abdou, and Mona E. Abass. 2021. Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. Alexandria Journal of Veterinary Sciences, 69 (2), 98-112. doi:10.5455/ajvs.83322



Chicago Style

Ebied, Nahla A., Mostafa S. Abdou, and Mona E. Abass. "Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate." Alexandria Journal of Veterinary Sciences 69 (2021), 98-112. doi:10.5455/ajvs.83322



MLA (The Modern Language Association) Style

Ebied, Nahla A., Mostafa S. Abdou, and Mona E. Abass. "Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate." Alexandria Journal of Veterinary Sciences 69.2 (2021), 98-112. Print. doi:10.5455/ajvs.83322



APA (American Psychological Association) Style

Ebied, N. A., Abdou, . M. S. & Abass, . M. E. (2021) Microbial Quality and Residues of Some Antibiotics in Conventional and Organic Table Eggs at Retail Stores in Kafrelsheikh Governorate. Alexandria Journal of Veterinary Sciences, 69 (2), 98-112. doi:10.5455/ajvs.83322





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