E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 90
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Original Article
Online Published: 29 Jul 2026
 


Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate

Hadeer Hany Mortada, Lamiaa Gamal Mostafa, Samy Abdelsalam Khalil.


Abstract
Foodborne diseases (FBDs) remain a significant public health challenge in Egypt, particularly concerning resilient spore-forming pathogens like Clostridium perfringens. This study investigated the prevalence and molecular profile of C. perfringens in 120 canned fish samples (60 mackerel; 60 sardine) retailed in Alexandria governorate between September 2024 and February 2025. Biochemical identification revealed a uniform prevalence of 6.67% (4/60) for both mackerel and sardine samples. Molecular surveillance using PCR identified the species-specific cpa gene in eight isolates and the enterotoxin-encoding cpe gene in six isolates (four mackerel; two sardine). Sequence analysis of the cpe amplicons showed high homology (95.07%–97.65%) to the C. perfringens strain A1 CPE enterotoxin gene. These findings indicate a breach of the Egyptian Standard No. 287/2005, which mandates the total absence of anaerobic spore-formers in canned seafood. The high genetic identity among isolates suggests potential endemic contamination within local manufacturing or handling environments. This study underscores the necessity for rigorous molecular monitoring to ensure the safety of ready-to-eat canned products.

Key words: Clostridium perfringens; cpe gene; Canned fish; Alexandria; PCR, gene sequencing


 
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How to Cite this Article
Pubmed Style

Mortada HH, Mostafa LG, Khalil SA. Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. AJVS. Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272

Web Style

Mortada HH, Mostafa LG, Khalil SA. Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. https://www.alexjvs.com/?mno=324272 [Access: July 30, 2026]. doi:10.5455/ajvs.324272

AMA (American Medical Association) Style

Mortada HH, Mostafa LG, Khalil SA. Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. AJVS. Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272


Vancouver/ICMJE Style

Mortada HH, Mostafa LG, Khalil SA. Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. AJVS, [cited July 30, 2026]; Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272


Harvard Style

Mortada, H. H., Mostafa, . L. G. & Khalil, . S. A. (0) Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. AJVS, Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272


Turabian Style

Mortada, Hadeer Hany, Lamiaa Gamal Mostafa, and Samy Abdelsalam Khalil. 0. Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. Alexandria Journal of Veterinary Sciences, Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272


Chicago Style

Mortada, Hadeer Hany, Lamiaa Gamal Mostafa, and Samy Abdelsalam Khalil. "Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate." Alexandria Journal of Veterinary Sciences Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272


MLA (The Modern Language Association) Style

Mortada, Hadeer Hany, Lamiaa Gamal Mostafa, and Samy Abdelsalam Khalil. "Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate." Alexandria Journal of Veterinary Sciences Online First: 29 Jul, 2026. Web. 30 Jul 2026 doi:10.5455/ajvs.324272


APA (American Psychological Association) Style

Mortada, H. H., Mostafa, . L. G. & Khalil, . S. A. (0) Molecular detection of certain enterotoxins of Clostridium perfringens isolated from canned fish retailed in Alexandria governorate. Alexandria Journal of Veterinary Sciences, Online First: 29 Jul, 2026. doi:10.5455/ajvs.324272




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Egypt.

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